Appendix B

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Note: Although this software and accompanying documentation is dated 2004-2005, it is still valid in 2014. Questions can be sent to CDC-INFO.

Template File Format

The FINAL.TPL file which describes a sample template and is distributed with other sample data files along with the program ELISA for Windows contains the following information:

8,”Test Template”
“S”,” 1″,” 1″,” 8″,” 3″,”600″,”2″,”L”,”3″,”H”,”Std 1″
“U”,” 1″,” 4″,” 2″,” 11″,”300″,”2″,”L”,”2″,”V”,”Unk 1″
“U”,” 3″,” 4″,” 4″,” 11″,”300″,”2″,”L”,”2″,”V”,”Unk 2″
“U”,” 5″,” 4″,” 6″,” 11″,”300″,”2″,”L”,”2″,”V”,”Unk 3″
“U”,” 7″,” 4″,” 8″,” 11″,”300″,”2″,”L”,”2″,”V”,”Unk 4″
“Q”,” 1″,” 12″,” 2″,” 12″,”1200″,”2″,”L”,”2″,”V”,”QC1″
“Q”,” 3″,” 12″,” 4″,” 12″,”1800″,”2″,”L”,”2″,”V”,”QC2″
“Q”,” 5″,” 12″,” 6″,” 12″,”19200″,”2″,”L”,”2″,”V”,”QC3″

From Module 2, the format for the 96-well ELISA plate which corresponds to the above file appears as in Figure 24.

Using this illustration, the FINAL.TPL file may be interpreted as follows: The first record contains a single integer which details how many lines follow in the rest of the file. The text to the right of this integer is the Project ID. There are eight lines of information, each of which defines one block of standards, unknowns or QC’s.

Each line describes one block and contains 11 fields. These fields are:

  1. Block category where S=standards; Q=quality control samples; and U=unknowns.
  2. The row number for the top left well in the block.
  3. The column number for the top left well in the block.
  4. The row number for the bottom right well in the block.
  5. The column number for the bottom right well in the block.
  6. The starting dilution in the series.
  7. The fold change or dilution factor.
  8. Indicator for dilution increase or decrease where L=low to high and H=high to low.
  9. The number of replicates in the block.
  10. “H” for horizontal or “V” for vertical. This details the orientation of the replicates in the block.
  11. The block ID.

As an example, the second line of file FINAL.TPL (the first line to follow the number 8) is information pertaining to a standard. The block of wells defining the standard range from row 1, column 1 to row 8, column 3. The initial dilution is 600; the dilution series is 2 fold and the dilutions proceed from low to high. There are three replicates per dilution which run horizontally. The block ID is ‘Std 1’.

Figure 24. Display of sample template design.

Figure 24. Display of sample template design.

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Page last reviewed: September 4, 2013