Instructions for Streptococcus Species Testing and Specimen Submission

What You Need to Know
  • CDC is requesting bacterial isolates and clinical specimens from pediatric patients with streptococcal brain abscesses or subdural or epidural empyemas.
  • Complete the 50.34 form for each submission and include a specimen ID.
  • Route all isolates and specimens through a state public health laboratory.

Instructions for diagnostic testing of clinical specimens

A standard diagnostic workup for children with brain abscesses should be done locally per treating clinicians.

Instructions for isolate and specimen submission

All bacterial isolates and residual culture-negative clinical specimens submitted to CDC for Streptococcus species testing must be routed through a state public health laboratory. All isolates and specimens must be shipped in accordance with all applicable local, state, and federal regulations.

If clinical specimens and/or bacterial isolates are available from cases, please have the microbiology laboratory hold and prepare to ship the following:

chart of laboratory instructions for isolate and specimen submission

To avoid a delay in testing, adhere to the following requirements:

  • Before submission, email CDCStrepInquiry@cdc.gov to inform CDC about the shipment, including number of samples together with specimen IDs being shipped.
  • Send bacterial isolate(s) and clinical specimen(s) to the CDC Streptococcus Lab with the accompanying 34 Specimen Submission Form according to instructions provided.
  • Complete the 50.34 form for each submission and include a specimen ID.
    • Specimens received without specimen IDs entered in the 50.34 form will not be processed for testing.

Storing and shipping bacterial isolates

Store pure bacterial isolates on an appropriate transport medium (e.g., blood, chocolate), or as a frozen glycerol culture (e.g., Tryptic Soy Broth with 15% glycerol, skim milk, tryptone, glucose, and glycerin (STGG) media).

Specimens can be stored at room temperature (15–25°C) if shipped within 24 hours and/or kept refrigerated (2–8°C) if shipped in less than 72 hours. Otherwise, specimens should be stored frozen (-20°C or lower) in a glycerol transport medium.

Ship frozen specimens on dry ice and refrigerated specimens on cold packs.

Storing and shipping clinical specimens

For clinical specimens, volume permitting, prepare one aliquot (minimum volume = 0.5 mL). Store and ship frozen (-20°C or lower).

Ship frozen specimens on dry ice and refrigerated specimens on cold packs.

Find additional information about the Streptococcus Study CDC-10217 Test Order.

Related Pages

Page last reviewed: June 8, 2022