Arbovirus Reference Collection (ARC) Contents and Maintenance

Contents

We Need Your Specimens to Improve Public Health

We’d like to collaborate with you! You can support public health by depositing your isolates in CDC’s Arbovirus Reference Collection (ARC). The ARC offers long-term curation, maintenance, and distribution of valuable isolates. Contact the ARC staff about depositing your isolates for long-term curation, maintenance, and distribution.

If you need reagents, visit our Arboviral Reagent Ordering System.

  • Isolates from environmental and human sources, dating back to 1925
  • Chimeric viruses
  • Antibody, antigen, and RNA controls for isolates in the collection
  • Hybridoma cells

Database Design

  • New database released in 2017
  • Relational database in Structured Query Language (SQL) to ensure data accuracy, reduce redundancy, and increase data security
  • Added features include:
    • Bibliography section
    • Product inserts
    • Increased functionality for recording quality control testing results, isolate history, and passage history and lineage during virus maintenance
    • Increased functionality for order maintenance
Yale Arbovirus Research Unit collection

Yale Arbovirus Research Unit collection housed at DVBD. Credit: CDC

Quality Control (QC) Testing

  • Viruses
    • Plaque titration to test for viability
    • Inclusivity and Exclusivity testing
    • Mycoplasma contamination testing
  • Antigen
    • Infectivity assay to confirm the material is non-infectious
    • Activity testing in limited assays, if available
  • Antibody
    • Activity testing in limited assays, if available
  • RNA
    • Activity testing in specific assays

Virus Maintenance

  • Track lineage
  • Minimize passage number
  • Perform phenotypic analysis
  • Compare growth in cell types now that suckling mouse brain is not the method of choice for replication
  • Obtain full genome with Next Generation Sequencing
    • Analysis and identification of unknown isolates and not fully characterized viruses
    • Expanded QC capabilities for historically maintained viruses