Water, Sanitation, and Hygiene Laboratories

The lead laboratory units for domestic and global water, sanitation, and hygiene (WASH)-related diseases associated with community settings and the natural environment in CDC’s National Center for Emerging and Zoonotic Infectious Diseases.

Our Environmental Microbiology and Engineering Laboratory Team and Clinical Detection and Surveillance Laboratory Team conduct:

  • Diagnostics for free-living ameba infections
  • Water-related outbreak investigations
  • Molecular surveillance for Giardia and Cryptosporidium
  • Water disinfection research
  • Serologic assay development

The teams also work with laboratories, subject matter experts, and programs around CDC.

Environmental Microbiology and Engineering Laboratory Team

Woman in lab doing on water disinfestation research
Water disinfection research

Woman in lab doing water sampling usingu ltrafiltration
Water sampling using ultrafiltration

Mission: Developing and applying methods for environmental sampling, microbial detection, and treatment technologies to prevent WASH-related disease.

Our laboratories:

  • Support water-related emergency preparedness and outbreak investigations both domestically and globally
  • Conduct environmental sampling and testing of water, wastewater, soil, sediment, biosolids, filters, and other environmental sample types
  • Quantify and/or detect viruses, bacteria, and parasites in environmental samples using culture, molecular, and microscopy methods
  • Develop and optimize environmental sample collection and microbial detection and characterization methods
  • Conduct environmental investigations to identify pathogens and indicators of fecal contamination
  • Develop and implement approaches for detectionand quantification of antibiotic resistance in the environment
  • Conduct primary research on the fate, transport, and disinfection mechanisms of waterborne pathogens in environmental systems
  • Assess disinfection and treatment methods for drinking water and recreational water
  • Provide tools for environmental microbiology laboratory capacity building and training on environmental sampling techniques and associated analytical methods

Environmental Microbiology and Engineering Laboratory Team Highlights

Clinical Detection and Surveillance Laboratory Team

Man in lavatory in front of a computer showing graphs
Molecular analysis of clinical and water samples
Man in lab looing into a microscope working on free-living amebas specimen analysis
Specimen analysis for free-living amebas

Mission: Developing and applying advanced molecular and cellular parasitology methods for clinical diagnostics, surveillance, and outbreak investigations to prevent WASH-related diseases.

Our laboratories:

  • Investigate the molecular and genomic epidemiology of waterborne and zoonotic parasites in humans and animals in the context of One Health
  • Molecularly subtype waterborne and foodborne protozoan pathogens (Cryptosporidium, Giardia, Naegleria fowleri, E. histolytica) in clinical, animal, and environmental samples
  • Develop novel molecular methods for diagnosing, characterizing, and differentiating predominantly waterborne parasites
  • Track sources and spread of protozoan parasites using advanced molecular detection tools, such as whole genome sequencing (WGS)
  • Diagnose amebic infections, including pathogenic free-living Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia
  • Develop and manage CryptoNet, CDC’s first molecular surveillance system for a parasite–a DNA sequence-based system for understanding U.S. transmission of cryptosporidiosis
  • Study waterborne parasite biology and Cryptosporidium oocyst production
  • Develop multiplex serologic assays for use in surveillance and epidemiologic surveys to understand the spread of WASH-related diseases

Clinical Detection and Surveillance Laboratory Team Highlights