Antimicrobial Susceptibility Testing Methods (AST)


This course provides a detailed overview of antimicrobial susceptibility testing methods including reference methods such as those described by the Clinical and Laboratory Standards Institute (CLSI) and commercial test systems. It focuses on technical and regulatory considerations for any method of medical laboratory antimicrobial susceptibility testing. The course provides essential information for those new to antimicrobial susceptibility testing and an important refresher for laboratory professionals with more experience.


At the conclusion of this eLearning, you will be able to:

  • Identify the steps required to perform the Clinical and Laboratory Standards Institute (CLSI) disk diffusion and Minimal Inhibitory Concentration (MIC) reference methods
  • Identify the key steps that must be modified when performing disk diffusion or MIC procedures for fastidious organisms
  • Review the importance and limitations of commercial antimicrobial susceptibility test systems
Continuing Education Units (CEU)

The Centers for Disease Control and Prevention, Division of Laboratory Systems, Laboratory Training and Services Branch, is approved as a provider of continuing education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program. This course is approved for 1.5 contact hours of P.A.C.E. ® credit.

P.A.C.E.® Course Number: 288-023-17

This course has been approved for 1.5 contact hours in the category of Microbiology/Mycology/Parasitology for Florida Laboratory Licensees.

Florida Course Number: 20-434651

More Information

Antimicrobial Susceptibility Testing Methodspdf icon

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Length of Course

1.5 Hours

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Credits Offered


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Delivery Method

On-demand eLearning

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Sponsoring Agency


Page last reviewed: October 27, 2017