03/21/2020: Lab Alert: Discontinue Use of the A549/MV1LU Mix for Respiratory Viral Culture Panels and Review Updated Biosafety FAQs

CDC's Laboratory Outreach Communication System (LOCS)

Audience: Clinical Laboratory Professionals

Level: Laboratory Alert

Discontinue Use of the A549/MV1LU Mix for Respiratory Viral Culture Panels and Review Updated Biosafety FAQs

Because of the risk of infection with SARS-CoV-2 among laboratory workers, CDC recommends that laboratories discontinue the use of the A549/MV1LU mix when they perform respiratory viral culture panels. Using these specific cell lines has been shown to result in a very low level of SARS-CoV replication. CDC recommends that laboratories perform respiratory viral culture panels using cell lines that will not support SARS-CoV viral replication.

Additionally, CDC has recently updated the biosafety guidelines and frequently asked questions (FAQs). According to the updated guidelines, cultures or isolates of SARS-CoV-2 may now be shipped according to regulations for UN 3373 Biological Substances, Category B.

Online resources:

If you have any questions, please contact us at LOCS@cdc.gov.

Laboratory Outreach Communication System | Division of Laboratory Systems (DLS)

Center for Surveillance, Epidemiology, and Laboratory Services (CSELS)

Centers for Disease Control and Prevention (CDC)

LOCS@cdc.gov

www.cdc.gov/csels/dls/locs

Page last reviewed: March 21, 2020