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Micronutrient and Nutrition-Related Publications


  • Chen H, McCoy LF, Schleicher RL, Pfeiffer CM. Measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method. Clin Chim Acta. 2008;391:6-12. Full Text
  • Erhardt JG, Estes JE, Pfeiffer CM, Biesalski HK, Craft NE. Combined measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein by an inexpensive, sensitive, and simple sandwich enzyme-linked immunosorbent assay technique. J Nutr. 2004 Nov;134(11):3127-32. Full Text
  • Fazili Z, Pfeiffer CM. Measurement of folates in serum and conventionally prepared whole blood lysates: application of an automated 96-well plate isotope-dilution tandem MS method. Clin Chem. 2004;50:2378-81. Full Text
  • Fazili Z, Pfeiffer CM, Zhang M, Jain R. Erythrocyte folate extraction and quantitative determination by liquid chromatography-tandem mass spectrometry: comparison of results with microbiologic assay. Clin Chem. 2005 Dec;51(12):2318-25. Full Text
  • McCoy LF, Bowen MB, Xu M, Chen H, Schleicher RL. Improved HPLC assay for measuring serum vitamin C with 1-methyluric acid used as an electrochemically active internal standard. Clin Chem. 2005 Jun;51(6):1062-4. Full Text
  • McCoy LF, Scholl PF, Schleicher RL, Groopman JD, Powers CD, Pfeiffer CM. Analysis of aflatoxin B1-lysine adduct in serum using isotope-dilution liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom. 2005;19(16):2203-10. Full Text
  • Nelson BC, Pfeiffer CM, Margolis SA, Nelson CP. Affinity extraction combined with stable isotope dilution LC/MS for the determination of 5-methyltetrahydrofolate in human plasma. Anal Biochem. 2003 Feb 1;313(1):117-27. Full Text
  • Nelson BC, Pfeiffer CM, Margolis SA, Nelson CP. Solid-phase extraction-electrospray ionization mass spectrometry for the quantification of folate in human plasma or serum. Anal Biochem. 2004 Feb 1;325(1):41-51. Full Text
  • Nelson BC, Pfeiffer CM, Sniegoski LT, Satterfield MB. Development and evaluation of an isotope dilution LC/MS method for the determination of total homocysteine in human plasma. Anal Chem. 2003 Feb 15;75(4):775-84. Full Text
  • Pawlosky RJ, Flanagan VP, Pfeiffer CM. Determination of 5-methyltetrahydrofolic acid in human serum by stable-isotope dilution high-performance liquid chromatography-mass spectrometry. Anal Biochem. 2001 Nov 15;298(2):299-305. Full Text
  • Pfeiffer CM, Fazili Z, McCoy LF, Gunter EW. Determination of folate vitamers in human serum by stable-isotope dilution tandem mass spectrometry and comparison to radioassay and microbiologic assay. Clin Chem. 2004;50(2):423-32. Full Text
  • Pfeiffer CM, Fazili Z, Zhang M. Folate analytical methodology. In: Bailey LB, editor. Folate in Health and Disease. 2nd ed. CRC Press. Taylor & Francis Group. 2010.
  • Pfeiffer CM, Huff DL, Gunter EW. Rapid and accurate HPLC assay for plasma total homocysteine and cysteine in a clinical laboratory setting. Clin Chem. 1999 Feb;45(2):290-2. Full Text
  • Pfeiffer CM, Twite D, Shih J, Holets-McCormack SR, Gunter EW. Method comparison for total plasma homocysteine between the Abbott IMx analyzer and an HPLC assay with internal standardization. Clin Chem. 1999 Jan;45(1):152-3. Full Text
  • Rybak ME, Parker DL, Pfeiffer CM. Determination of urinary phytoestrogens by HPLC-MS/MS: a comparison of atmospheric pressure chemical ionization (APCI) and electrospray ionization (ESI). J Chromat B 2008;861:145-50. Full Text
  • Rybak ME, Pfeiffer CM. Clinical analysis of vitamin B6: Determination of pyridoxal 5′-phosphate and 4-pyridoxic acid in human serum by reversed-phase high-performance liquid chromatography with chlorite postcolumn derivatization. Anal Biochem. 2004;333:336-44. Full Text
  • Rybak ME, Pfeiffer CM. A simplified protein precipitation and filtration procedure for determining serum vitamin B(6) by high-performance liquid chromatography. Anal Biochem 2009 May 1;388(1):175-7. Full Text
  • Satterfield MB, Sniegoski LT, Welch MJ, Nelson BC, Pfeiffer CM. Comparison of isotope dilution mass spectrometry methods for the determination of total homocysteine in plasma and serum. Anal Chem. 2003 Sep 1;75(17):4631-8. Full Text

Method Comparisons

  • Blanck HM, Pfeiffer CM, Caudill SP, Reyes R, Gunter EW, Imperatore G. Serum iron and iron-binding capacity: a round-robin interlaboratory comparison study. Clin Chem. 2003; 49:1672-75. Full Text
  • Caldwell KL, Maxwell CB, Makhmudov A, Pino S, Braverman LE, Jones RL, Hollowell JG. Use of inductively coupled plasma mass spectrometry to measure urinary iodine in NHANES 2000: comparison with previous method. Clin Chem 2003 Jun;49(6 Pt 1):1019-21. Full Text
  • Dearth-Wesley T, Makhmudov A, Pfeiffer CM, Caldwell CM. Fast and reliable salt iodine measurement: evaluation of the WYD Iodine Checker in comparison with iodometric titration. Food and Nutrition Bulletin. 2004:25(2):130-36. Full Text
  • Fazili Z, Pfeiffer CM, Zhang M. Comparison of serum folate species analyzed by LC-MS/MS with total folate measured by microbiologic assay and bio-rad radioassay. Clin Chem. 2007;53(4):781-84. Full Text
  • Fazili Z, Pfeiffer CM, Zhang M, Jain RB, Koontz D. Influence of 5,10-methylenetetrahydrofolate reductase polymorphism on whole blood folate concentrations measured by LC-MS/MS, microbiologic assay, and BioRad radioassay. Clin Chem. 2008;54:197-201. Full Text
  • La’ulu SL, Rawlins ML, Pfeiffer CM, Zhang M, Roberts WL. Performance characteristics of six homocysteine assays. Am J Clin Pathol. 2008 Dec;130(6):969-75. Full Text
  • McCoy LF, Scholl PF, Sutcliffe AE, Kieszak SM, Powers CD, Rogers HS, Gong YY, Groopman JD, Wild CP, Schleicher RL. Human aflatoxin albumin adducts quantitatively compared by ELISA, HPLC with fluorescence detection, and HPLC with isotope dilution mass spectrometry. Cancer Epidemiol Biomarkers Prev. 2008 Jul;17(7):1653-7. Full Text
  • Rybak ME, Jain RB, Pfeiffer CM. Clinical vitamin B6 anlaysis: an inter-laboratory comparison of pyridoxal 5′-phosphate measurements in serum. Clin Chem. 2005;51(7):1223-31. Full Text
  • Pfeiffer CM, Cook JD, Mei Z, Cogswell ME, Looker AC, Lacher DA. Evaluation of an automated soluble transferrin receptor (sTfR) assay on the Roche Hitachi analyzer and its comparison to two ELISA assays. Clin Chim Acta. 2007 Jul;382(1-2):112-6. Full Text
  • Pfeiffer CM, Huff DL, Smith SJ, Miller DT, Gunter EW. Comparison of plasma total homocysteine measurements in 14 laboratories: an international study. Clin Chem. 1999;45(8):1261-68. Full Text
  • Pfeiffer CM, Smith SJ, Miller DT, Gunter EW. Comparison of serum and plasma methylmalonic acid measurements in 13 laboratories: An international study. Clin Chem. 1999 Dec;45(12):2236-42. Full Text
  • Zhang M, Gunter EW, Pfeiffer CM. Evaluation of the Drew Scientific ds30 homocysteine assay in comparison with the Centers for Disease Control and Prevention reference HPLC method. Clin Chem. 2001 May;47(5):966-7. Full Text
  • Zhang M, Pfeiffer CM. Comparing the ESA HPLC total homocysteine assay with electrochemical detection to the CDC in-house HPLC assay with fluorescence detection. Clin Chim Acta. 2004 Feb;340(1-2):195-200. Full Text


  • Caldwell KL, Jones RL, Hollowell JG. Urinary iodine concentration: United States National Health and Nutrition Examination Survey 2001-2002. Thyroid. 2005;15(7):692-99. Full Text
  • Caldwell KL, Miller GA, Wang RY, Jain RB, Jones RL. Iodine status of the U.S. population, national health and nutrition examination survey 2003-2004. Thyroid 2008 Nov;18(11):1207-14. Full Text
  • Cogswell ME, Looker AC, Pfeiffer CM, Cook JD, Lacher DA, Beard JL, Lynch SR, Grummer-Strawn LM. Assessment of iron deficiency in US preschool children and nonpregnant females of childbearing age: National Health and Nutrition Examination Survey 2003-2006. Am J Clin Nutr. 2009 May;89(5):1334-42. Epub 2009 Apr 8. Full Text
  • Ford ES, Schleicher RL, Mokdad AH, Ajani UA, Liu S. Distribution of serum concentrations of alpha-tocopherol and gamma-tocopherol in the US population. Am J Clin Nutr. 2006 Aug;84(2):375-83. Full Text
  • Hollowell JG, Staehling NW, Hannon WH, Flanders DW, Gunter EW, Maberly GF, Braverman LE, Pino S, Miller DT, Garbe PL, DeLozier DM, Jackson RJ. Iodine nutrition in the United States. Trends and public health implications: iodine excretion data from National Health and Nutrition Examination Surveys I and III (1971-1974 and 1988-1994). J Clin Endocrinol Metab. 1998 Oct;83(10):3401-8. Full Text
  • Looker AC, Pfeiffer CM, Lacher DA, Schleicher RL, Picciano MF, Yetley EA. Serum 25-hydroxyvitamin D status of the US population: 1988-1994 compared with 2000-2004. Am J Clin Nutr. 2008 Dec;88(6):1519-27. Full Text
  • Pfeiffer CM, Caudill SP, Gunter EW, Bowman BA, Jacques PF, Selhub J, Johnson CL, Miller DT, Sampson EJ. Analysis of factors influencing the comparison of homocysteine values between the Third National Health and Nutrition Examination Survey (NHANES) and NHANES 1999+. J Nutr. 2000 Nov;130(11):2850-4. Full Text
  • Pfeiffer CM, Caudill SP, Gunter EW, Osterloh J, Sampson EJ. Biochemical indicators of B vitamin status in the U.S. population after folic acid fortification: results from the National Health and Nutrition Examination Survey 1999-2000. Am J Clin Nutr. 2005;82:442-50. Full Text
  • Pfeiffer CM, Johnson CL, Jain RB, Yetley EA, Picciano MF, Rader JI, Fisher KD, Mulinare J, Osterloh JD. Trends in blood folate and vitamin B12 concentrations in the United States, 1988-2004. Am J Clin Nutr. 2007;86:718-27. Full Text
  • Pfeiffer CM, Osterloh JD, Kennedy-Stephenson J, Picciano MF, Yetley EA, Rader JI, Johnson CL. Trends in circulating concentrations of total homocysteine among U.S. adolescents and adults: findings from the 1991-1994 and 1999-2004 National Health and Nutrition Examination Surveys. Clin Chem. 2008;54:801-13. Full Text
  • Schleicher RL, Carroll MD, Ford ES, Lacher DA. Serum vitamin C and the prevalence of vitamin C deficiency in the United States: 2003-2004 National Health and Nutrition Examination Survey (NHANES). Am J Clin Nutr 2009 Nov;90(5):1252-63. Full Text


  • Aufreiter S, Gregory JF III, Pfeiffer CM, Fazili Z, Kim YI, Marcon N, Kamalaporn P, Pencharz PB, O’Connor DL. Folate is absorbed across the colon of adults: evidence from cecal infusion of (13)C-labeled [6S]-5-formyltetrahydrofolic acid. Am J Clin Nutr 2009 Jul;90(1):116-23. Full Text
  • Dorgan JF, Boakye NA, Fears TR, Schleicher RL, Helsel W, Anderson C, Robinson J, Guin JD, Lessin S, Ratnasinghe LD, Tangrea JA. Serum carotenoids and alpha-tocopherol and risk of nonmelanoma skin cancer. Cancer Epidemiol Biomarkers Prev. 2004 Aug;13(8):1276-82. Full Text
  • Hertrampf E, Cortés F, Erickson JD, Cayazzo M, Freire W, Bailey LB, Howson C, Kauwell GP, Pfeiffer C. Consumption of folic acid-fortified bread improves folate status in women of reproductive age in Chile. J Nutr. 2003 Oct;133(10):3166-9. Full Text
  • Huang LL, Coleman HR, Kim J, de Monasterio F, Wong WT, Schleicher RL, Ferris FL, Chew EY, et al. Oral supplementation of lutein/zeaxanthin and omega-3 long chain polyunsaturated fatty acids in persons aged 60 years or older, with or without AMD. Invest Opthalmol Vis Sci. 2008;49:3864-69. Full Text
  • Kelvin EA, Edwards S, Jedrychowski W, Schleicher RL, Camann D, Tang D, Perera FP. Modulation of the effect of prenatal PAH exposure on PAH-DNA adducts in cord blood by plasma antioxidants. Cancer Epidemiol Biomarkers Prev. 2009 Aug;18(8):2262-8. Full Text
  • Limburg PJ, Wei W, Ahnen DJ, Qiao Y, Hawk ET, Wang G, Giffen CA, Wang G, Roth MJ, Lu N, Korn EL, Ma Y, Caldwell KL, Dong Z, Taylor PR, Dawsey SM. Randomized, placebo-controlled, esophageal squamous cell cancer chemoprevention trial of selenomethionine and celecoxib. Gastroenterology 2005 Sep;129(3):863-73. Full Text
  • Lindblad AS, Lloyd PC, Clemons TE, Gensler GR, Ferris FL 3rd, Klein ML, Armstrong JR; Age-Related Eye Disease Study Research Group. Change in area of geographic atrophy in the Age-Related Eye Disease Study: AREDS report number 26. Arch Ophthalmol. 2009 Sep;127(9):1168-74. Full Text
  • Looker HC, Fagot-Campagna A, Gunter EW, Pfeiffer CM, Narayan KM, Knowler WC, Hanson RL. Homocysteine as a risk factor for nephropathy and retinopathy in Type 2 diabetes. Diabetologia. 2003 Jun;46(6):766-72. Full Text
  • Looker HC, Fagot-Campagna A, Gunter EW, Pfeiffer CM, Sievers ML, Bennett PH, et al. Homocysteine and vitamin B12 concentrations and mortality rates in type 2 diabetes. Diabetes Metab Res Rev. 2007;23:193-201. Full Text
  • Luo M, Estívariz CF, Schleicher RL, Bazargan N, Leader LM, Galloway JR, Ziegler TR. Prospective analysis of serum carotenoids, vitamin A, and tocopherols in adults with short bowel syndrome undergoing intestinal rehabilitation. Nutrition. 2009 Apr;25(4):400-7. Full Text
  • Mark SD, Qiao Y-L, Dawsey SM, Wu Y-P, Katki H, Gunter EW, Fraumeni JF Jr., Blot WJ, Dong Z-W, Taylor PR, et al. Prospective Study of Serum Selenium Levels and Incident Esophageal and Gastric Cancers. Journal of the National Cancer Institute. 2000; 92(21):1753-63. Full Text
  • Masters ET, Jedrychowski W, Schleicher RL, Tsai WY, Tu YH, Camann D, Tang D, Perera FP, et al. Relation between prenatal lipid-soluble micronutrient status, environmental pollutant exposure, and birth outcomes. Am J Clin Nutr. 2007;86:1139-45. Full Text
  • Nouarie M, Pourshams A, Kamangar F, Sotoudeh M, Derakhshan MH, Akbari MR, Fakheri H, Zahedi MJ, Caldwell K, Abnet CC, Taylor PR, Malekzadeh R, Dawsey SM. Ecologic study of serum selenium and upper gastrointestinal cancers in Iran. World J Gastroenterol 2004 Sep 01;10(17):2544-6. Full Text
  • Rosenthal JM, Kim J, de Monasterio F, Thompson DJ, Bone RA, Landrum JT, de Moura FF, Khachik F, Chen H, Schleicher RL, Ferris FL 3rd, Chew EY. Dose-ranging study of lutein supplementation in persons aged 60 years or older. Invest Ophthalmol Vis Sci. 2006 Dec;47(12):5227-33. Erratum in: Invest Ophthalmol Vis Sci. 2007 Jan;48(1):17. de Monastario, Francisco [corrected to de Monasterio, Francisco]. Full Text
  • Rosenthal J, Milla G, Flores A, Yon M, Pfeiffer CM, Umana E, Scarrett N, Barahona F, The Cooperative Folic Acid Research Group, et al. Effect of different dosage and administration schedules of folic acid on blood folate levels in a population of Honduran women of reproductive age. Pub Health Nutr. 2008;11:822-30. Full Text
  • Sutter ME, Thomas JD, Caldwell KL, Makhmudov A, Morgan BW. Selenosis from nutritional supplement formulation error. Clin Toxicol. 2008 Aug 01;46(7):598.
  • Taren DL, Duncan B, Shrestha K, Shrestha N, Genaro-Wolf D, Schleicher RL, Pfeiffer CM, Sowell AL, Greivenkamp J, Canfield L. The night vision threshold test is a better predictor of low serum vitamin A concentration than self-reported night blindness in pregnant urban Nepalese women. J Nutr. 2004 Oct;134(10):2573-8. Full Text
  • Wolff MS, Teitelbaum SL, Windham G, Pinney SM, Britton JA, Chelimo C, Godbold J, Biro F, Kushi LH, Pfeiffer CM, Calafat AM, et al. Pilot study of urinary biomarkers of phytoestrogens, phthalates, and phenols in girls. Environ Health Perspect. 2007;115:116-21. Full Text


  • Caldwell CL, Makmudov A, Jones RL, Hollowell JG. EQUIP: a worldwide program to ensure the quality of urinary iodine procedures. Accred Qual Assur. 2005;10(7):356-61. Full Text
  • Caudill SP, Schleicher RL, Pirkle JL. Multi-rule quality control for the age-related eye disease study. Stat Med. 2008 Sep 10;27(20):4094-106. Full Text
  • Drammeh BS, Schleicher RL, Pfeiffer CM, Jain RB, Zhang M, Nguyen PH. Effects of delayed specimen processing and freezing on serum concentrations of selected nutritional indicators. Clin Chem. 2008;54:1883-91. Full Text
  • Haynes BMH, Schleicher RL, Jain RB, Pfeiffer CM. The CDC VITAL-EQA program, external quality assurance for serum retinol 2003-2006. Clin Chim Acta. 2008;390:90-96. Full Text
  • NACB LMPG Committee members, Myers GL, Christenson RH, Cushman M, Ballantyne CM, Cooper GR, Pfeiffer CM, Grundy SM, Labarthe DR, Levy D, Rifai N, Wilson PW. National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: emerging biomarkers for primary prevention of cardiovascular disease. Clin Chem 2009 Feb;55(2):378-84. Full Text
  • Nelson BC, Pfeiffer CM, Zhang M, Duewer DL, Sharpless KE, Lippa KA. Commutability of NIST SRM 1955 homocysteine and folate in frozen human serum with selected total homocysteine immunometri and enzymatic assays. Clin Chim Acta. 2008;395:99-105. Full Text
  • Pearce EN. U.S. Iodine Nutrition: Where Do We Stand? Thyroid. 2008;18(11):1143-45. Full Text
  • Satterfield MB, Sniegoski LT, Sharpless KE, Welch MJ, Hornikova A, Zhang NF, Pfeiffer CM, Fazili Z, Zhang M, Nelson BC. Development of a new standard reference material: SRM 1955 (homocysteine and folate in human serum). Anal Bioanal Chem. 2006 Jun;385(3):612-22. Full Text
  • Thorpe SJ, Heath A, Blackmore S, Lee A, Hamilton M, O’Broin S, Nelson BC, Pfeiffer CM, et al. International standard for serum vitamin B12 and serum folate: international collaborative study to evaluate a batch of lyophilized serum for B12 and folate content. Clin Chem Lab Med. 2007;45:380-86. Full Text