AMD: Improving Vaccines

Vaccines have helped eliminate many diseases in the United States, such as polio, but other vaccine-preventable diseases, like whooping cough (pertussis), are rebounding. Using AMD technology, CDC scientists can find shifts in the DNA of circulating bacteria and viruses that help them evade vaccines. Through this work, we can develop more effective vaccines that shift with the changing microbes.

Fighting Vaccine-preventable Disease Outbreaks
A nurse in the process of administering an intramuscular vaccination into a girl’s left arm whose sister, mother, and grandmother watched from the background

Vaccine-preventable diseases, such as measles, mumps, rubella, and polio, can easily mutate to evade vaccines. Investigators are using AMD technologies to develop a viral genomics pipeline (VGP) to better track the sources of outbreaks, in the United States and worldwide. VGP combines standardized laboratory methods with user-friendly computer analyses to detect previously unknown viruses faster and accelerate vaccine development and improvement. CDC is sharing the VGP with state public health laboratories to improve state capacity to respond to important disease threats.

Finding DNA Shifts in Pertussis

Despite the availability of vaccines against pertussis (whooping cough), the disease has rebounded to between 10,000 and 50,000 reported cases in the United States each year since 2010. Using AMD technology, CDC scientists have found shifts in the DNA of circulating Bordetella pertussis bacteria that could be contributing to the resurgence of pertussis. One bacterial component targeted by the vaccine, pertactin, is a protein believed to help the bacteria attach to cells in the throat. However, researchers have confirmed that the types of B. pertussis that cause the most illness in the United States are missing pertactin. With AMD methods, investigators are mapping the complete genome of historical and currently circulating strains of B. pertussis to determine if these genetic changes are contributing to the reemergence of pertussis.

Improving Influenza Vaccines

Developing the annual flu shot is a year-round process that involves surveillance and characterization of circulating flu viruses, decisions by global influenza experts regarding which viruses should be included in the upcoming season’s vaccine, production and growth of candidate vaccine viruses used in manufacturing,  and the manufacture of the vaccines in time for flu season.

With AMD, the characterization of flu viruses has been greatly enhanced, giving experts better data faster to support vaccine decisions.

figure showing the difference between amd methods and flu vaccineimage icon[JPG - 160 KB]
Better Flu Surveillance

Older genetic sequencing methods require specimens taken from patients to be grown in culture to create a virus isolate that can then be sequenced. With AMD, specimens can be sequenced directly, without first growing them in culture to create a virus isolate. That time-saving benefit has reduced the response time to flu outbreaks and with CDC’s innovative approach it’s resulting in better, cheaper data in less time.

Page last reviewed: July 3, 2019