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The mission of the One Test. Two Lives. campaign from the Centers for Disease Control and Prevention (CDC) is to help prevent transmission of human immunodeficiency virus (HIV) from mother to child and promote optimal maternal health.

The One Test. Two Lives. campaign focuses on ensuring that all women are tested for HIV early in their pregnancy and gives obstetric providers new tools to help ensure all patients get tested for HIV early in their pregnancy.

One Test. Two Lives. provides quick access to a variety of resources for providers, and materials for their patients, to help encourage universal voluntary prenatal testing for HIV.

Even if a mother has HIV, her baby doesn’t have to. Advances in HIV treatment have greatly lowered the chances that a mother will pass HIV on to her baby. Women living with HIV who get and stay in treatment can reduce their baby’s risk of infection to 1% or less.

Inform Patients

Download easy-to-read, brief patient education materials about HIV and the benefits of prenatal HIV testing.

Get Resource Kit

Access this information kit that includes CDC recommendations for HIV testing, provider and patient materials, and a due date projection wheel with HIV testing reminders as examples.

Order Free Campaign Materials

Order or download fact sheets, pamphlets, posters, and educational materials at CDC-INFO OnDemand.

Find Information and Tools for Providers

Learn more about preventing perinatal infection, as well as HIV treatment options.

Letter from the Director

Headshot of Dr. Eugene McCray We are almost there! Today, pregnant women living with human immunodeficiency virus (HIV) can reduce the risk of perinatal transmission to 1% or less by taking antiretroviral medicines as prescribed during pregnancy. However, perinatal HIV transmission cannot be eliminated if pregnant women are not tested for HIV and linked to care if HIV-positive. Identifying new cases is critical to stopping perinatal transmission of HIV. The Centers for Disease Control and Prevention (CDC) and the American College of Obstetricians and Gynecologists recommend that obstetricians test all pregnant patients for HIV, preferably in the first trimester.


Get Tested

Find an HIV testing site near you.


For more information, please visit

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