Isolation of Diverse Simian Arteriviruses Causing Hemorrhagic Disease
Teressa M. Shaw, Samuel T. Dettle, Andres Mejia, Jennifer M. Hayes, Heather A. Simmons, Puja Basu, Jens H. Kuhn, Mitchell D. Ramuta, Cody J. Warren, Peter B. Jahrling, David H. O’Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I. Slukvin, Matthew E. Brown, and Adam L. Bailey
Author affiliations: University of Wisconsin, Madison, Wisconsin, USA (T.M. Shaw, S.T. Dettle, A. Mejia, M.D. Ramuta, D.H. O’Connor, L. Huang, M. Zaeem, J. Seo, I.I. Slukvin, M.E. Brown, A.L. Bailey); Wisconsin National Primate Research Center, Madison (S.T. Dettle, A. Mejia, J.M. Hayes, H.A. Simmons, P. Basu, D.H. O’Connor, I.I. Slukvin); National Institutes of Health, Fort Detrick, Frederick, Maryland, USA (J.H. Kuhn, P.B. Jahrling); The Ohio State University, Columbus, Ohio, USA (C.J. Warren)
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Figure 9
![Ct values for virus-specific nucleoprotein RNA in mice engrafted with rhesus monkey immune cells infected with diverse simarteriviruses causing hemorrhagic disease. NSG-SGM3 (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg[CMV-IL3,CSF2,KITLG]1Eav/MloySzJ) mice were engrafted with fetal CD34+-enriched hematopoietic stem and progenitor cells isolated from rhesus monkeys. Engrafted and unengrafted NSG-SGM3 transgenic mice and background-matched wild-type NOR/LtJ mice were injected intraperitoneally and intravenously with 1 of 4 viruses: A) simian hemorrhagic fever virus; B) Kibale red colobus monkey virus 1; C) Pebjah virus; or D) Southwest baboon virus 1. Production of virus-specific nucleoprotein RNA in blood was measured over time by using quantitative reverse transcription PCR. Dotted lines indicates limit of detection. Ct, cycle threshold.](/eid/images/23-1457-F9.jpg)
Figure 9. Ct values for virus-specific nucleoprotein RNA in mice engrafted with rhesus monkey immune cells infected with diverse simarteriviruses causing hemorrhagic disease. NSG-SGM3 (NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg[CMV-IL3,CSF2,KITLG]1Eav/MloySzJ) mice were engrafted with fetal CD34+-enriched hematopoietic stem and progenitor cells isolated from rhesus monkeys. Engrafted and unengrafted NSG-SGM3 transgenic mice and background-matched wild-type NOR/LtJ mice were injected intraperitoneally and intravenously with 1 of 4 viruses: A) simian hemorrhagic fever virus; B) Kibale red colobus monkey virus 1; C) Pebjah virus; or D) Southwest baboon virus 1. Production of virus-specific nucleoprotein RNA in blood was measured over time by using quantitative reverse transcription PCR. Dotted lines indicates limit of detection. Ct, cycle threshold.
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