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Volume 30, Number 4—April 2024
Research

Isolation of Diverse Simian Arteriviruses Causing Hemorrhagic Disease

Teressa M. Shaw, Samuel T. Dettle, Andres Mejia, Jennifer M. Hayes, Heather A. Simmons, Puja Basu, Jens H. Kuhn, Mitchell D. Ramuta, Cody J. Warren, Peter B. Jahrling, David H. O’Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I. Slukvin, Matthew E. Brown, and Adam L. BaileyComments to Author 
Author affiliations: University of Wisconsin, Madison, Wisconsin, USA (T.M. Shaw, S.T. Dettle, A. Mejia, M.D. Ramuta, D.H. O’Connor, L. Huang, M. Zaeem, J. Seo, I.I. Slukvin, M.E. Brown, A.L. Bailey); Wisconsin National Primate Research Center, Madison (S.T. Dettle, A. Mejia, J.M. Hayes, H.A. Simmons, P. Basu, D.H. O’Connor, I.I. Slukvin); National Institutes of Health, Fort Detrick, Frederick, Maryland, USA (J.H. Kuhn, P.B. Jahrling); The Ohio State University, Columbus, Ohio, USA (C.J. Warren)

Main Article

Figure 8

Ct values for virus-specific nucleoprotein RNA in macrophages infected with diverse simarteriviruses causing hemorrhagic disease. Induced pluripotent stem cell–derived macrophages from crab-eating macaques were infected with different simarteriviruses. Virus-specific nucleoprotein RNA was measured by using quantitative reverse transcription PCR on different days after inoculation. Dotted lines indicate limit of detection. Error bars indicate SEMs. Ct, cycle threshold; dpi, days after inoculation; iPSC, induced pluripotent stem cell; KRCV-1, Kibale red colobus monkey virus 1; LoD, limit of detection; PBJV, Pebjah virus; SHFV, simian hemorrhagic fever virus; SWBV-1, Southwest baboon virus 1.

Figure 8. Ct values for virus-specific nucleoprotein RNA in macrophages infected with diverse simarteriviruses causing hemorrhagic disease. Induced pluripotent stem cell–derived macrophages from crab-eating macaques were infected with different simarteriviruses. Virus-specific nucleoprotein RNA was measured by using quantitative reverse transcription PCR on different days after inoculation. Dotted lines indicate limit of detection. Error bars indicate SEMs. Ct, cycle threshold; dpi, days after inoculation; iPSC, induced pluripotent stem cell; KRCV-1, Kibale red colobus monkey virus 1; LoD, limit of detection; PBJV, Pebjah virus; SHFV, simian hemorrhagic fever virus; SWBV-1, Southwest baboon virus 1.

Main Article

Page created: February 15, 2024
Page updated: March 20, 2024
Page reviewed: March 20, 2024
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