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Volume 30, Number 4—April 2024
Research

Isolation of Diverse Simian Arteriviruses Causing Hemorrhagic Disease

Teressa M. Shaw, Samuel T. Dettle, Andres Mejia, Jennifer M. Hayes, Heather A. Simmons, Puja Basu, Jens H. Kuhn, Mitchell D. Ramuta, Cody J. Warren, Peter B. Jahrling, David H. O’Connor, Liupei Huang, Misbah Zaeem, Jiwon Seo, Igor I. Slukvin, Matthew E. Brown, and Adam L. BaileyComments to Author 
Author affiliations: University of Wisconsin, Madison, Wisconsin, USA (T.M. Shaw, S.T. Dettle, A. Mejia, M.D. Ramuta, D.H. O’Connor, L. Huang, M. Zaeem, J. Seo, I.I. Slukvin, M.E. Brown, A.L. Bailey); Wisconsin National Primate Research Center, Madison (S.T. Dettle, A. Mejia, J.M. Hayes, H.A. Simmons, P. Basu, D.H. O’Connor, I.I. Slukvin); National Institutes of Health, Fort Detrick, Frederick, Maryland, USA (J.H. Kuhn, P.B. Jahrling); The Ohio State University, Columbus, Ohio, USA (C.J. Warren)

Main Article

Figure 1

Simarterivirus infection of grivet embryonic kidney cell line in study of isolation of diverse simarteriviruses causing hemorrhagic disease. MA-104 cells were inoculated with SHFV at a multiplicity of infection of 1 and inoculated with KRCV-1, PBJV, or SWBV-1 by using sample volumes equivalent to that of SHFV (n = 3 experiments for each virus). Supernatants were collected at the indicated timepoints and quantitative reverse transcription PCR was used to measure simarterivirus nucleoprotein gene levels. Dashed line indicates limit of detection. Only SHFV replicated in grivet MA-104 cells. Ct, cycle threshold; KRCV-1, Kibale red colobus monkey virus 1; PBJV, Pebjah virus; SHFV, simian hemorrhagic fever virus; SWBV-1, Southwest baboon virus 1.

Figure 1. Simarterivirus infection of grivet embryonic kidney cell line in study of isolation of diverse simarteriviruses causing hemorrhagic disease. MA-104 cells were inoculated with SHFV at a multiplicity of infection of 1 and inoculated with KRCV-1, PBJV, or SWBV-1 by using sample volumes equivalent to that of SHFV (n = 3 experiments for each virus). Supernatants were collected at the indicated timepoints and quantitative reverse transcription PCR was used to measure simarterivirus nucleoprotein gene levels. Dashed line indicates limit of detection. Only SHFV replicated in grivet MA-104 cells. Ct, cycle threshold; KRCV-1, Kibale red colobus monkey virus 1; PBJV, Pebjah virus; SHFV, simian hemorrhagic fever virus; SWBV-1, Southwest baboon virus 1.

Main Article

Page created: February 15, 2024
Page updated: March 20, 2024
Page reviewed: March 20, 2024
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