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Identification of other Streptococcus Species: Streptococcus General Methods

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The reference used for compiling the methods in Section I is:
Murray, P.R., Baron, E. J., Jorgensen, J.J., Pfaller, M.A., and Yolken, R.H. Manual of Clinical Microbiology, 8th ed. ASM Press: Washington, DC, 2003.

The Streptococcus species identification methods in Section II were compiled by several individuals, including Lynn Shewmaker, Richard Facklam, and Lucia Teixeira.

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Section I.

  1. Accuprobe-Enterococcus Test
  2. Accuprobe-Pneumococcus Test
  3. Acid formation in carbohydrate broth
  4. Arginine Hydrolysis
  5. Bacitracin Test
  6. Bile-esculin Test
  7. Bile solubility Test
  8. CAMP Test
  9. Catalase Test
  10. Clindamycin test
  11. Esculin hydrolysis
  12. Gas from MRS broth
  13. Gram Stain
  14. Growth at 10 & 45C
  15. Hemolysis
  16. Hippurate hydrolysis
  17. Lancefield Group Antigen
  18. Leucine amino peptidase (LAP)
  19. Litmus Milk Test
  20. Motility
  21. 6.5% NaCl Tolerance Test
  22. Optochin
  23. Pigmentation
  24. Pyridoxal Requirement Test (Vitamin B6)
  25. Pyrrolidonlarylamindase (PYR)
  26. Pyruvate Utilization Test
  27. Satellite Test
  28. Starch hydrolysis
  29. Sucrose agar (extracellular polysaccharides)
  30. Sucrose broth (5%)
  31. Tellurite tolerance
  32. Urea hydrolysis
  33. Vancomycin test
  34. Voges-Proskauer

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Section II.

Identification Procedures for Catalase-Negative Gram-positive Cocci

A. General Description of the general identified in this lab

Table 1.
Identification of gram-positive general Beta-hemolytic streptococci
Table 2.
Identification and reporting of β-hemolytic streptococci
Table 3.
Identification of atypical group A strep
Table 4.
Identification of the S. anginous group
Non-beta-hemolytic streptococci
Streptococcus pneumoniae
Table 5.
Identification non-β-hemolytic strep
Table 6.
Characteristics of viridans streptococci
Table 7.
Identification of nutritionally variant strep

B. Identification of Enterococcus and Vagococcus

Table 8.
Identification of Enterococcus and Vagococcus species

C. Identification of Lactococcus species

Table 9.
Identification of Lactococcus species

D. Identification of Gemella species

Table 10.
Identification of Gemella species

E. Identification of Pediococcus and Tetragenococcus species

Table 11.
Identification of Pediococcus and Tetragenococcus

F. Identification of Leuconostoc species

Table 12.
Identification of Leuconostoc species

G. Identification of Aerococcus and Helococcus

Table 13.
Identification of Aerococcus and Helococcus

H. Identification of Globicatella

Table 14.
Identification of Globicatella

I. Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus

Table 15.
Identification of Dolosigranulum, Ignavigranum, Facklamia, and Alloicococcus
Table 16.
Rapid ID-32 for unusual gram positive bacteria

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