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Gonorrhea Laboratory Information
Neisseria meningitidis

Introduction

The primary purpose of this page is to provide illustrations of characteristics of N. meningitidis that may aid in differentiating between this, and other, Neisseria species that produce acid from glucose and maltose.

This page is not intended to be a definitive discussion of N. meningitidis infections but to provide information relating to the accurate identification of N. meningitidis and will include only traditional tests for the identification of this species, information on species that may be misidentified as N. meningitidis, and additional tests which should be performed to identify a gram-negative, oxidase-positive diplococcal strain accurately.

For information about meningococcal meningitis, visit the Meningococcal Disease page, Division of Bacterial and Mycotic Diseases.

Table 1. Characteristics of N. meningitidis

Characteristic Illustration
Gram stain
Cell Morphology
Gram-negative diplococcus
Colony Morphology colonies
Pigmentation pigmentation
Oxidase Test Oxidase
Acid Production acid
Enzyme Substrate Test Gamma-glutamyl aminopeptidase +ve
Nitrate Reduction Test Nitrate -ve
Polysaccharide from Sucrose

Although strains of some organisms do not grow on medium on which polysaccharide is detected, polysaccharide may be detected in the growth inoculated onto the plate. N. meningitidis does not produce polysaccharide from sucrose.

polysaccharide
Polysaccharide -ve
Production of
Deoxyribonuclease (DNase)
DNAse
DNase -ve
Superoxol Test
Reaction with 30% hydrogen peroxide
superoxol
Strain variable
Weak (1+) to Strong (4+)
Catalase Test
Reaction with 3% hydrogen peroxide
catalase
Catalase-positive
Colistin Resistance colistin
Colistin-resistant

Neisseria species may be misidentified as N. meningitidis in acid detection tests. Supplemental tests may be used to differentiate between them.

Table 2. Differential characteristics of Neisseria spp. which produce acid from glucose and maltose.

Species Acid from Enzyme Substrate
Polysaccharide
from Sucrose
Pigment Colistin
Resistance
G M S F L
N. meningitidis + + - - - Gammaglutamyl-
aminopeptidase +ve
- - R
N. polysaccharea + + - - - Hydroxyprolyl-
aminopeptidase +ve
+ - (R)
N. sublava
Biovar subflava
Biovar flava*
+ + - - - Hydroxyprolyl-
aminopeptidase +ve
OR
Gammaglutamyl-
aminopeptidase +ve
- + (R)
Lactose-negative
N. lactamica**
+ + - +/- - Beta-
galactosidase +ve
- - R

*N. subflava biovar flava will be identified as N. subflava biovar subflava if acid production from fructose is not determined.
**The author has encountered one lactose-negative strain of N. lactamica; this strain was identified with an enzyme substrate test which demonstrated that the organism produced beta-galactosidase.

Although enzyme substrate tests are intended to be used only for the identification of Neisseria spp. isolated on selective media for N. gonorrhoeae, isolates of other Neisseria spp. are gamma-glutamylaminopeptidase-positive in this test as are isolates of N. meningitidis. Thus, additional tests must be performed to differentiate between these species.

Table 3. Supplemental tests which permit differentiation among Neisseria and related species that produce gamma-glutamylaminopeptidase.

Species that Produce
gamma-glutamylamino-
peptidase
Acid from Polysaccharide
from Sucrose
Colistin
Resistance
Pigment
G M S F L
N. meningitidis + + - - - - R Pink-brown
N. subflava
biovar subflava
+ + - - - - (R) Yellow
N. subflava
biovar flava
+ + - + - - S Yellow
N. subflava
biovar perflava
+ + + + - + (R) Yellow

Abbreviations: +, most strains positive; -, most strains negative; R, strains grow well on selective medium for N. gonorrhoeae and/or show no inhibition around a colistin disk (10 micrograms); (R), most strains susceptible, some strains known to be resistant; S, strains susceptible, no strains known to be resistant.


References

Bovre K. 1984. Family VIII. Neisseriaceae Prevot, p. 288-309. In N. R. Krieg (ed.). Manual of Systematic Bacteriology, vol. 1. The Williams & Wilkins co., Baltimore.

Knapp, J. S. 1988. Historical perspectives and identification of Neisseria and related species. Clin. Microbiol. Rev. 1:415-431.

Knapp JS, Rice RJ. Neisseria and Branhamella. In. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. (ed.). Manual of Clinical Microbiology. 6th ed. American Society for Microbiology, Washington D. C, 1995.

Vedros NA. 1984. Genus I. Neisseria Trevisan 1885, 105AL, p. 290-296. In N. R. Krieg (ed.). Bergey's Manual of Systematic Bacteriology, vol. 1. The Williams & Wilkins Co., Baltimore.

 

 
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