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Appendix F2 - Figure 1

Supplement F: Laboratory Guidance

Public Health Guidance for Community-Level Preparedness and Response to Severe Acute Respiratory Syndrome (SARS) Version 2/3

Figure 1 - SARS-CoV Specimen Testing Guidelines: RT-PCR Testing

RT-PCR testing chart, see text version below

Text version of Figure 1

For Respiratory specimens; Blood, plasma, or serum; and Stool

Prepare four aliquots1

  1. To test for SARS-CoV
  2. For repeat testing
  3. For confirmatory testing
  4. To test for other agents

Store remaining sample.


  • If Neg result, specimen is Neg for SARS-CoV.2
    • Testing for other pathogens may be indicated.
  • If control guidelines are not met, repeat test using new aliquot.
  • If Pos result, repeat test using new aliquot.
    • If Neg result, additional testing may be advised.2
    • If Pos result x2, this is presumptive identification of SARS-CoV.
      • Send aliquot to CDC for confirmatory testing. Include copy of data.


  1. Preparation of aliquots must be done where there is no risk of contamination.
  2. A negative result does not rule out SARS-CoV infection. See Appendix F7 for more information.


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