Skip directly to search Skip directly to A to Z list Skip directly to navigation Skip directly to site content Skip directly to page options
CDC Home

Pathogens & Protocols

PulseNet detects subtypes of E. coli O157 and other Shiga toxin-producing E. coli, Campylobacter jejuni, Clostridium botulinum*, Listeria monocytogenes, Salmonella, Shigella, Vibrio cholerae, and Vibrio parahaemolyticus.

PulseNet Pathogens & Protocols

Pulsed Field Gel Electrophoresis (PFGE) and Multiple Locus Variable Number tandem repeat Analysis (MLVA) are the two main subtyping (or “fingerprinting”) tools used by PulseNet. Due to differences inherent to the bacteria that PulseNet subtypes, PulseNet has developed separate protocols for different bacteria for PFGE and MLVA. Additionally, because of the variation between machines, MLVA protocols have been developed for different machine platforms as well. These protocols are listed below and can also be found listed on each pathogen page.

*Clostridium botulinum is considered a select agent and, as such, follows different handling procedures than other pathogens covered by PulseNet.

 
Contact Us:
  • Centers for Disease Control and Prevention
    1600 Clifton Rd
    Atlanta, GA 30333
  • 800-CDC-INFO
    (800-232-4636)
    TTY: (888) 232-6348
  • Contact CDC-INFO
USA.gov: The U.S. Government's Official Web PortalDepartment of Health and Human Services
Centers for Disease Control and Prevention   1600 Clifton Rd. Atlanta, GA 30333, USA
800-CDC-INFO (800-232-4636) TTY: (888) 232-6348 - Contact CDC–INFO
A-Z Index
  1. A
  2. B
  3. C
  4. D
  5. E
  6. F
  7. G
  8. H
  9. I
  10. J
  11. K
  12. L
  13. M
  14. N
  15. O
  16. P
  17. Q
  18. R
  19. S
  20. T
  21. U
  22. V
  23. W
  24. X
  25. Y
  26. Z
  27. #