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Pathogens & Protocols

PulseNet detects subtypes of E. coli O157 and other Shiga toxin-producing E. coli, Campylobacter jejuni, Clostridium botulinum*, Listeria monocytogenes, Salmonella, Shigella, Vibrio cholerae, Vibrio parahaemolyticus, and Cronobacter.

PulseNet Pathogens & Protocols

Pulsed Field Gel Electrophoresis (PFGE) and Multiple Locus Variable Number tandem repeat Analysis (MLVA) are the two main subtyping (or “fingerprinting”) tools used by PulseNet. Due to differences inherent to the bacteria that PulseNet subtypes, PulseNet has developed separate protocols for different bacteria for PFGE and MLVA. Additionally, because of the variation between machines, MLVA protocols have been developed for different machine platforms as well. These protocols are listed below and can also be found listed on each pathogen page.

*Clostridium botulinum is considered a select agent and, as such, follows different handling procedures than other pathogens covered by PulseNet.

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