The aim of the study was to develop a new indicator sensor pad for detection of glutaraldehyde permeation of chemical protective gloves. The pad carries a reagent which responds to glutaraldehyde contaminant by producing a color change. Some commonly used glutaraldehyde and alkaline glutaraldehyde solutions, Metricide®, Cetylcide-G®, and 50% glutaraldehyde solution, were analyzed by solvent desorption and gas chromatography. All glutaraldehyde solutions exhibited >98% adsorption on the pads over the spiking range 0.05-5.0 µL. Recovery for each glutaraldehyde solution was calculated, ranging from 58-92% (RSD =4.0%) for all glutaraldehyde solutions. Breakthrough times for two protective glove materials (PVC and polymerized alkene) were determined using the Thermo-Hand Method, and found to range from 76 to 150 min for Metricide®, from 170 to 230 min for Cetylcide-G®, and from 232 to 300 min for 50% glutaraldehyde. The quantitative mass of the glutaraldehyde solutions on the pads at the time of breakthrough detection ranged from 35-37, 37-39, and 38-40 µg/cm² for Metricide®, Cetylcide-G®, and 50% glutaraldehyde, respectively. The new aldehyde indicator pad should find utility in detecting, collecting, and quantitative analyzing glutaraldehyde and alkaline glutaraldehyde permeation samples in the workplace.