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Production of a Chaetomium globosum enolase monoclonal antibody.

Authors
Green-BJ; Nayak-AP; Lemons-AR; Rittenour-WR; Hettick-JM; Beezhold-DH
Source
Monoclon Antib Immunodiagn Immunother 2014 Dec; 33(6):428-437
NIOSHTIC No.
20045555
Abstract
Chaetomium globosum is a hydrophilic fungal species and a contaminant of water-damaged building materials in North America. Methods to detect Chaetomium species include subjective identification of ascospores, viable culture, or molecular-based detection methods. In this study, we describe the production and initial characterization of a monoclonal antibody (MAb) for C. globosum enolase. MAb 1C7, a murine IgG1 isotype MAb, was produced and reacted with recombinant C. globosum enolase (rCgEno) in an enzyme-linked immunosorbent assay and with a putative C. globosum enolase in a Western blot. Epitope mapping showed MAb 1C7 specific reactivity to an enolase decapeptide, LTYEELANLY, that is highly conserved within the fungal class Sordariomycetes. Cross-reactivity studies showed MAb 1C7 reactivity to C. atrobrunneum but not C. indicum. MAb 1C7 did not react with enolase from Aspergillus fumigatus, which is divergent in only two amino acids within this epitope. The results of this study suggest potential utility of MAb 1C7 in Western blot applications for the detection of Chaetomium and other Sordariomycetes species.
Keywords
Fungi; Molecular-biology; Molds; Bacteria; Construction-materials; Antibody-response
Contact
B. J. Green, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
Publication Date
20141211
Document Type
Journal Article
Email Address
dox6@cdc.gov
Fiscal Year
2015
NTIS Accession No.
NTIS Price
Issue of Publication
6
ISSN
2167-9436
NIOSH Division
HELD
Priority Area
Services
Source Name
Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
State
WV
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