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Effects of DNA repair gene polymorphisms on DNA damage in human lymphocytes induced by a vinyl chloride metabolite in vitro.

Authors
Feng-N; Li-Y; Long-C; Xia-Z-L; Brandt-Rauf-PW
Source
Biomarkers 2014 Jun; 19(4):281-286
NIOSHTIC No.
20045510
Abstract
Background: Epidemiologic studies suggest that variability in DNA damage from vinyl chloride monomer (VCM) may be partially mediated by genetic polymorphisms in DNA repair. This study aimed to corroborate these observations with controlled experiments in vitro using cell lines from individuals with differing DNA repair genotypes to determine damage following VCM metabolite exposure. Methods: Matched pairs of lymphoblast cell lines (homozygous wild-type versus homozygous variant for either XRCC1 399 or XPD 751 polymorphism) were exposed to chloroacetaldehyde and analyzed by the cytokinesis-block micronucleus assay. Results: All cell lines demonstrated a dose-response of increasing micronuclei with increasing exposure, but for both XRCC1 and XPD, the polymorphic cells peaked at higher micronucleus frequencies and declined at a slower rate to baseline than the wild-type cells. Conclusion: This supports the findings that XRCC1 and XPD polymorphisms may result in deficient DNA repair of VCM-induced genetic damage.
Keywords
Epidemiology; DNA-damage; Monomers; In-vitro-study; Cell-biology; Cell-function; Cellular-function; Cell-metabolism; Metabolism; Genes; Exposure-levels; Metabolites; Morphology; Author Keywords: DNA repair; micronuclei; polymorphisms
Contact
Dr. Paul W. Brandt-Rauf, Dean's Office, School of Public Health, University of Illinois at Chicago, 1603 West Taylor Street, Chicago, IL 60612
CODEN
BIOMFA
CAS No.
75-01-4; 107-20-0; 9002-86-2
Publication Date
20140601
Document Type
Journal Article
Email Address
pwb1@uic.edu
Funding Type
Grant
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-004192
Issue of Publication
4
ISSN
1354-750X
Source Name
Biomarkers
State
IL
Performing Organization
University of Illinois
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