Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Endotoxin deposits on the inner surfaces of closed-face cassettes during bioaerosol sampling: a field investigation at composting facilities.

Authors
Duquenne-P; Simon-X; Demange-V; Harper-M; Wild-P
Source
Ann Occup Hyg 2015 May; 59(4):504-513
NIOSHTIC No.
20045496
Abstract
A set of 270 bioaerosol samples was taken from 15 composting facilities using polystyrene closed-face filter cassettes (CFCs). The objective was to measure the quantity of endotoxin deposits on the inner surfaces of the cassettes (sometimes referred to as 'wall deposits'). The results show that endotoxins are deposited on the inner surfaces of the CFCs through sampling and/or handling of samples. The quantity of endotoxins measured on inner surfaces range between 0.05 (the limit of detection of the method) and 3100 endotoxin units per cassette. The deposits can represent a large and variable percentage of the endotoxins sampled. More than a third of the samples presented a percentage of inner surface deposits >40% of the total quantity of endotoxins collected (filter + inner surfaces). Omitting these inner surface deposits in the analytical process lead to measurement errors relative to sampling all particles entering the CFC sampler, corresponding to a developing consensus on matching the inhalable particulate sampling convention. The result would be underestimated exposures and could affect the decision as to whether or not a result is acceptable in comparison to airborne concentration limits defined in terms of the inhalability convention. The results of this study suggest including the endotoxins deposited on the inner surfaces of CFCs during analysis. Further researches are necessary to investigate endotoxin deposits on the inner cassette surfaces in other working sectors.
Keywords
Endotoxins; Waste-treatment; Waste-disposal; Biohazards; Aerosols; Samplers; Sampling; Sampling-equipment; Author Keywords: air sampling; bioaerosol; closed-face cassette; composting facilities; endotoxins; inner surfaces deposits; wall deposits
Contact
Philippe Duquenne, INRS - Institut National de Recherche et de Sécurité, Aerosols Metrology Laboratory, Pollutants Metrology Division, 1 rue du Morvan CS 60027, Vandoeuvre-lès-Nancy Cedex 54519, France
CODEN
AOHYA3
Publication Date
20150501
Document Type
Journal Article
Email Address
philippe.duquenne@inrs.fr
Fiscal Year
2015
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0003-4878
NIOSH Division
HELD
Source Name
Annals of Occupational Hygiene
State
WV
TOP