Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Detection and measurement of surface contamination by multiple antineoplastic drugs using multiplex bead assay.

Authors
Smith-JP; Sammons-DL; Robertson-SA; Pretty-JR; DeBord-DG; Connor-TH; Snawder-JE
Source
J Oncol Pharm Pract 2016 Feb; 22(1):60-67
NIOSHTIC No.
20045274
Abstract
OBJECTIVES: Contamination of workplace surfaces by antineoplastic drugs presents an exposure risk for healthcare workers. Traditional instrumental methods to detect contamination such as liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) are sensitive and accurate but expensive. Since immunochemical methods may be cheaper and faster than instrumental methods, we wanted to explore their use for routine drug residue detection for preventing worker exposure. METHODS: In this study we examined the feasibility of using fluorescence covalent microbead immunosorbent assay (FCMIA) for simultaneous detection and semi-quantitative measurement of three antineoplastic drugs (5-fluorouracil, paclitaxel, and doxorubicin). The concentration ranges for the assay were 0-1000 ng/ml for 5-fluorouracil, 0-100 ng/ml for paclitaxel, and 0-2 ng/ml for doxorubicin. The surface sampling technique involved wiping a loaded surface with a swab wetted with wash buffer, extracting the swab in storage/blocking buffer, and measuring drugs in the extract using FCMIA. RESULTS: There was no significant cross-reactivity between these drugs at the ranges studied indicated by a lack of response in the assay to cross analytes. The limit of detection (LOD) for 5-fluorouracil on the surface studied was 0.93 ng/cm2 with a limit of quantitation (LOQ) of 2.8 ng/cm2, the LOD for paclitaxel was 0.57 ng/cm2 with an LOQ of 2.06 ng/cm2, and the LOD for doxorubicin was 0.0036 ng/cm2 with an LOQ of 0.013 ng/cm2. CONCLUSION: The use of FCMIA with a simple sampling technique has potential for low cost simultaneous detection and semi-quantitative measurement of surface contamination from multiple antineoplastic drugs.
Keywords
Antineoplastic-agents; Oncogenic-agents; Bioassays; Work-environment; Environmental-contamination; Immunochemistry; Drugs; Detectors; Measurement-equipment; Sampling; Sampling-methods; Quantitative-analysis; Analytical-processes; Author Keywords: Antineoplastic drugs; 5-fluorouracil; paclitaxel; doxorubicin; surface analysis; multiplex measurement
Contact
Jerome P Smith, Division of Applied Research and Technology, National Institute for Occupational Safety and Health, US Centers for Disease Control and Prevention, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
CODEN
JOPPFI
CAS No.
51-21-8; 33069-62-4; 23214-92-8
Publication Date
20160201
Document Type
Journal Article
Email Address
jps3@cdc.gov
Fiscal Year
2016
NTIS Accession No.
NTIS Price
Identifying No.
M102014
Issue of Publication
1
ISSN
1078-1552
NIOSH Division
DART
Source Name
Journal of Oncology Pharmacy Practice
State
OH
TOP