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The penetratin sequence in the anticancer PNC-28 peptide causes tumor cell necrosis rather than apoptosis of human pancreatic cancer cells.

Authors
Bowne-WB; Sookraj-KA; Vishnevetsky-M; Adler-V; Sarafraz-Yazdi-E; Lou-S; Koenke-J; Shteyler-V; Ikram-K; Harding-M; Bluth-MH; Ng-M; Brandt-Rauf-PW; Hannan-R; Bradu-S; Zenilman-ME; Michl-J; Pincus-MR
Source
Ann Surg Oncol 2008 Dec; 15(12):3588-3600
NIOSHTIC No.
20045169
Abstract
BACKGROUND: PNC-27 and PNC-28 are p53-derived peptides from the human double minute (hdm-2) binding domain attached to penetratin. These peptides induce tumor cell necrosis of cancer cells, but not normal cells. The anticancer activity and mechanism of PNC-28 (p53 aa17-26-penetratin) was specifically studied against human pancreatic cancer. METHODS: MiaPaCa-2 cells were treated with PNC-28. Necrosis was determined by measuring lactate dehydrogenase (LDH) and apoptosis as assayed for measuring elevation of proapoptotic proteins. PNC-29, an unrelated peptide, and hdm-2-binding domain p53 aa12-26 without penetratin (PNC-26) were used as controls. Since there is evidence that penetratin is required for tumor cell necrosis, we tested "naked" p53 peptide without penetratin by transfecting a plasmid that encodes p53 aa17-26 segment of PNC-28 into MiaPaCa-2 and an untransformed rat pancreatic acinar cell line, BMRPA1. Time-lapse electron microscopy was employed to further elucidate anticancer mechanism. RESULTS: Treatment with PNC-28 does not result in the elevation of proapoptotic proteins found in p53-induced apoptosis, but elicits rapid release of LDH, indicative of tumor cell necrosis. Accordingly, we observed membrane pore formation and dose-dependent killing. In direct contrast, transfected MiaPaCa-2 cells underwent apoptosis, and not necrosis, as evidenced by expression of high levels of caspases-3 and 7 and annexin V with background levels of LDH. CONCLUSION: These results suggest that PNC-28 may be effective in treating human pancreatic cancer. The penetratin sequence appears to be responsible for the fundamental change in the mechanism of action, inducing rapid necrosis initiated by membrane pore formation. Cancer cell death by apoptosis was observed in the absence of penetratin.
Keywords
Peptides; Tumors; Cell-function; Cell-biology; Cellular-function; Cancer; Pancreatic-islets-neoplasms; Proteins; Drugs; Pharmacology; Metabolism; Pathology
Contact
Wilbur B. Bowne MD, Department of Surgery, SUNY Downstate Medical Center, 450 Clarkson Avenue, Brooklyn, NY, 11203
CODEN
ASONF4
Publication Date
20081201
Document Type
Journal Article
Email Address
wilbur.bowne@va.gov
Funding Type
Grant
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-007590
Issue of Publication
12
ISSN
1068-9265
Priority Area
Research Tools and Approaches: Cancer Research Methods
Source Name
Annals of Surgical Oncology
State
NY
Performing Organization
Columbia University Health Sciences
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