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Validation of an HPLC-MS/MS and wipe procedure for mitomycin C contamination.

Authors
B'Hymer-CB; Connor-T; Stinson-D; Pretty-J
Source
J Chromatogr Sci 2015 Apr; 53(4):619-624
NIOSHTIC No.
20045056
Abstract
A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the determination of mitomycin C, an anticancer drug, from contamination on various surfaces. Mitomycin C is often used in various forms of intraperitoneal chemotherapy, and operating room healthcare worker exposure to this drug is possible. The surface testing method consisted of a wiping procedure utilizing a solution of 20/45/35 (v/v/v) of acetonitrile-isopropanol-water made 0.01 M in ammonium citrate (apparent pH 7.0). The wipe solutions were analyzed by means of HPLC-MS/MS using a reversed-phase gradient system and electrospray ionization in positive ion mode with a triple-quadrupole MS detector. Accuracy and precision of this method were demonstrated by a series of recovery studies of both spiked solutions and extracted wipes from various surfaces (stainless steel, vinyl and FormicaŽ) spiked with known levels of mitomycin C. Recoveries of spiked solutions containing the analyte demonstrate mean recoveries (accuracy) ranged from 93 to 105%. Precision as measured by the relative standard deviation (% RSD) of multiple samples (n= 10) at each concentration level demonstrated values of 7.5% or less. The recoveries from spiked surfaces varied from 30 to 99%. The limit of detection for this methodology is approximately 2 ng/100 cm2 equivalent surface area, and the limit of quantitation is approximately 6 ng/100 cm2.
Keywords
Chromatographic-analysis; Cancer; Drugs; Chemotherapy; Antineoplastic-agents; Analytical-processes; Analytical-methods
Contact
Clayton B'Hymer, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226
CODEN
JCHSBZ
CAS No.
75-05-8; 67-63-0
Publication Date
20150401
Document Type
Journal Article
Email Address
cbhymer@cdc.gov
Fiscal Year
2015
NTIS Accession No.
NTIS Price
Identifying No.
M092014
Issue of Publication
4
ISSN
0021-9665
NIOSH Division
DART
Priority Area
Services
Source Name
Journal of Chromatographic Science
State
OH
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