Validation of an HPLC-MS/MS and wipe procedure for mitomycin C contamination.
B'Hymer-CB; Connor-T; Stinson-D; Pretty-J
J Chromatogr Sci 2015 Apr; 53(4):619-624
A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method was developed for the determination of mitomycin C, an anticancer drug, from contamination on various surfaces. Mitomycin C is often used in various forms of intraperitoneal chemotherapy, and operating room healthcare worker exposure to this drug is possible. The surface testing method consisted of a wiping procedure utilizing a solution of 20/45/35 (v/v/v) of acetonitrile-isopropanol-water made 0.01 M in ammonium citrate (apparent pH 7.0). The wipe solutions were analyzed by means of HPLC-MS/MS using a reversed-phase gradient system and electrospray ionization in positive ion mode with a triple-quadrupole MS detector. Accuracy and precision of this method were demonstrated by a series of recovery studies of both spiked solutions and extracted wipes from various surfaces (stainless steel, vinyl and FormicaŽ) spiked with known levels of mitomycin C. Recoveries of spiked solutions containing the analyte demonstrate mean recoveries (accuracy) ranged from 93 to 105%. Precision as measured by the relative standard deviation (% RSD) of multiple samples (n= 10) at each concentration level demonstrated values of 7.5% or less. The recoveries from spiked surfaces varied from 30 to 99%. The limit of detection for this methodology is approximately 2 ng/100 cm2 equivalent surface area, and the limit of quantitation is approximately 6 ng/100 cm2.
Chromatographic-analysis; Cancer; Drugs; Chemotherapy; Antineoplastic-agents; Analytical-processes; Analytical-methods
Clayton B'Hymer, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Taft Laboratory C-23, 4676 Columbia Parkway, Cincinnati, OH 45226
Journal of Chromatographic Science