Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Cytotoxicity and characterization of particles collected from an indium-tin oxide production facility.

Authors
Badding-MA; Stefaniak-AB; Fix-NR; Cummings-KJ; Leonard-SS
Source
J Toxicol Environ Health, A 2014 Oct; 77(20):1193-1209
NIOSHTIC No.
20045028
Abstract
Occupational exposure to indium compound particles has recently been associated with lung disease among workers in the indium-tin oxide (ITO) industry. Previous studies suggested that excessive alveolar surfactant and reactive oxygen species (ROS) may play a role in the development of pulmonary lesions following exposure to indium compounds. However, toxicity at the cellular level has not been comprehensively evaluated. Thus, the aim of this study was to assess which, if any, compounds encountered during ITO production are toxic to cultured cells and ultimately contribute to the pathogenesis of indium lung disease. The compounds used in this study were collected from eight different processing stages at an ITO production facility. Enhanced dark field imaging showed 5 of the compounds significantly associated with cells within 1 h, suggesting that cellular reactions to the compound particles may be occurring rapidly. To examine the potential cytotoxic effects of these associations, ROS generation, cell viability, and apoptosis were evaluated following exposures in RAW 264.7 mouse monocyte macrophage and BEAS-2B human bronchial epithelial cell lines. Both exhibited reduced viability with exposures, while apoptosis only occurred in RAW 264.7 cells. Our results suggested that excessive ROS production is likely not the predominant mechanism underlying indium-induced lung disease. However, the effects on cell viability reveal that several of the compounds are cytotoxic, and therefore, exposures need to be carefully monitored in the industrial setting.
Keywords
Cytotoxicity; Cytotoxic-effects; Tin-compounds; Tin-oxides; Indium-compounds; Oxides; Respiratory-system-disorders; Pulmonary-system-disorders; Lung-disorders; Lung-cells; Alveolar-cells; Lung-lesions; Metal-workers; Metallic-compounds; Metalworking-industry; Cell-cultures; Cell-culture-techniques; Cellular-reactions; Laboratory-animals; Laboratory-testing; Exposure-assessment; Industrial-exposures; Humans; Surfactants; Oxidative-processes; Cell-damage
Contact
Melissa A. Badding, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Mail Stop 2015, Morgantown, WV 26505-2888, USA
CODEN
JTEHD6
CAS No.
7440-74-6; 64815-01-6; 71243-84-0; 7440-31-5; 18282-10-5
Publication Date
20141001
Document Type
Journal Article
Email Address
wid5@cdc.gov
Fiscal Year
2015
NTIS Accession No.
NTIS Price
Identifying No.
M092014
Issue of Publication
20
ISSN
1528-7394
NIOSH Division
HELD; DRDS
Priority Area
Manufacturing
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV
TOP