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A mitochondrial pathway for biosynthesis of lipid mediators.

Authors
Tyurina-YY; Poloyac-SM; Tyurin-VA; Kapralov-AA; Jiang-J; Anthonymuthu-TS; Kapralova-VI; Vikulina-AS; Jung-M-Y; Epperly-MW; Mohammadyani-D; Klein-Seetharaman-J; Jackson-TC; Kochanek-PM; Pitt-BR; Greenberger-JS; Vladimirov-YA; Bayir-H; Kagan-VE
Source
Nat Chem 2014 Jun; 6(6):542-552
NIOSHTIC No.
20044800
Abstract
The central role of mitochondria in metabolic pathways and in cell-death mechanisms requires sophisticated signalling systems. Essential in this signalling process is an array of lipid mediators derived from polyunsaturated fatty acids. However, the molecular machinery for the production of oxygenated polyunsaturated fatty acids is localized in the cytosol and their biosynthesis has not been identified in mitochondria. Here we report that a range of diversified polyunsaturated molecular species derived from a mitochondria-specific phospholipid, cardiolipin (CL), is oxidized by the intermembrane-space haemoprotein, cytochrome c. We show that a number of oxygenated CL species undergo phospholipase A2-catalysed hydrolysis and thus generate multiple oxygenated fatty acids, including well-known lipid mediators. This represents a new biosynthetic pathway for lipid mediators. We demonstrate that this pathway, which includes the oxidation of polyunsaturated CLs and accumulation of their hydrolysis products (oxygenated linoleic, arachidonic acids and monolysocardiolipins), is activated in vivo after acute tissue injury.
Keywords
Animals; Laboratory-animals; Brain-function; Brain-matter; Drugs; Metabolism; Chemical-reactions; Pharmacology; Oxidation
CODEN
NCAHBB
Publication Date
20140601
Document Type
Journal Article
Funding Type
Grant
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282; M082014
Issue of Publication
6
ISSN
1755-4330
Source Name
Nature Chemistry
State
PA
Performing Organization
University of Pittsburgh at Pittsburgh
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