Urinary bisphenol A concentrations and cytochrome P450 19 A1 (Cyp19) gene expression in ovarian granulosa cells: an in vivo human study.
Ehrlich-S; Williams-PL; Hauser-R; Missmer-SA; Peretz-J; Calafat-AM; Flaws-JA
Reprod Toxicol 2013 Dec; 42:18-23
BACKGROUND: Exposure to bisphenol A (BPA), a chemical widely used in consumer products, has been associated with in vitro Cyp19 gene expression. OBJECTIVE: To evaluate an in vivo human model of Cyp19 gene expression in granulosa cells. STUDY DESIGN: A subset of an ongoing prospective cohort study of women undergoing in vitro fertilization (IVF) at Massachusetts General Hospital. METHODS: Mixed effect models were used to evaluate the association of urinary BPA concentrations with granulosa cell Cyp19 mRNA expression. RESULTS: In 61 women undergoing 76 IVF cycles, adjusted changes in mean Cyp19 expression (beta estimate (95% CI)) for quartiles 2, 3 and 4 as compared to the lowest quartile were: -0.97 (-2.22, 0.28); -0.97 (-2.18, 0.24) and -0.38 (-1.58, 0.82). CONCLUSIONS: An in vivo model for evaluation of Cyp19 gene expression was developed for use in epidemiologic studies. In this pilot study, we found no statistically significant linear association between urinary BPA concentrations and Cyp19 expression.
Humans; Women; Reproductive-system; Fertility; Urinalysis; Analytical-models; Phenols; Toxic-materials; Toxicology; Statistical-analysis; Genes; Cytology; In-vivo-study; Cell-function; Cellular-reactions; Cell-metabolism; Ribonucleic-acids; Models;
Author Keywords: Bisphenol A; Cyp19; Fertility; Follicular fluid; Gene expression; Human; Ovary granulosa cells; P450 aromatase
Russ Hauser, Harvard School of Public Health, 665 Huntington Avenue, Building I, 14th Floor, Boston, MA 02115, USA
Harvard University, School of Public Health, Boston, Massachusetts