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Luciferase reporter cells as a platform to detect SMAD-dependent collagen production.

Authors
Lohcharoenkal-W; Liu-Y; Wang-L; Yang-Y; Rojanasakul-Y
Source
J Biosci Bioeng 2014 Dec; 118(8):732-735
NIOSHTIC No.
20044667
Abstract
Aberrant collagen production can lead to many diseases such as fibrosis. Current methods of collagen detection are insensitive, time-consuming and laborious. We have developed a rapid, sensitive assay using chemiluminescence-based reporter cell system. Stable 3T3/NIH-SMAD-luciferase cells were generated for detection of collagen expression through TGF- signaling, a major fibrogenic pathway. We demonstrated that these reporter cells could be used as a rapid screening tool for detection of SMAD-dependent collagen production with higher sensitivity than existing assays. Flexibility of this cell-based assay in different detection platforms makes it attractive for high throughput screening of potential fibrogenic agents and drug candidates.
Keywords
Screening-methods; Bioassays; Luminescence; Cytology; Diagnostic-tests; Analytical-methods; Fibrosis; Fibrogenicity; Cell-function; Cellular-reactions; Medical-screening; Biochemical-indicators; Author Keywords: Luciferase; Reporter cells; TGF-; Collagen; Fibrosis
Contact
Yon Rojanasakul, West Virginia University, Department of Pharmaceutical Sciences, Mary Babb Randolph Cancer Center, Morgantown, WV 26506
CODEN
JBBIF6
Publication Date
20141201
Document Type
Journal Article
Email Address
yrojan@hsc.wvu.edu
Fiscal Year
2015
NTIS Accession No.
NTIS Price
Identifying No.
M072014
Issue of Publication
8
ISSN
1389-1723
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Journal of Bioscience and Bioengineering
State
WV
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