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Phage-displayed peptide that mimics aflatoxins and its application in immunoassay.

Authors
Wang-Y; Wang-H; Li-P; Zhang-Q; Kim-HJ; Gee-SJ; Hammock-BD
Source
J Agric Food Chem 2013 Mar; 61(10):2426-2433
NIOSHTIC No.
20044636
Abstract
To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 g/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 g/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis. Erratum: J Agric Food Chem 2013 Apr; 61(16):3994
Keywords
Analytical-processes; Fungi; Toxins; Microorganisms; Author Keywords: Aflatoxin; phage-displayed peptide; phage peptide library; mimotope; matrix effect
Contact
Peiwu Li, Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, P. R. China
CODEN
JAFCAU
Publication Date
20130313
Document Type
Journal Article
Email Address
peiwuli@oilcrops.cn
Funding Type
Cooperative Agreement
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007550
Issue of Publication
10
ISSN
0021-8561
Source Name
Journal of Agricultural and Food Chemistry
State
CA
Performing Organization
University of California - Davis
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