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Extended tracking of the microbial community structure and dynamics in an industrial synthetic metalworking fluid system.

Authors
Kapoor-R; Selvaraju-SB; Yadav-JS
Source
FEMS Microbiol Ecol 2014 Mar; 87(3):664-677
NIOSHTIC No.
20044569
Abstract
Understanding of the occupational exposure risk scenario and disease etiology associated with industrial metalworking fluids (MWFs) requires knowledge of the development and composition of their microbial diversity in relation to the underlying fluid management factors. In this study, a managed synthetic MWF operation freshly recharged following the dumping, cleaning, and recharge (DCR) process was tracked in real time for microbial community changes over a period of 1.25 years (65 weeks). The recharged fluid developed very high bacterial counts (viable and nonviable) fairly quickly after the DCR process, indicating its inadequacy. Genus-/group-specific real-time qPCR confirmed the prevalence of six potentially pathogenic/immunogenic microbial genera/groups, viz. pseudomonads, enterics, mycobacteria, legionellae, actinomycetes, and fungi. Selective culturing revealed Acinetobacter and Bacillus as the most frequently isolated Gram-negative and Gram-positive genera, respectively, in addition to the presence of fungi and actinomycetes. Endotoxin perturbations (< 1000 to > 100000 EU mL?) coincided with temporal increases in Gram-negative bacteria and/or periodic biocide additions. PCR-DGGE-sequencing revealed an expanded estimated bacterial richness (up to 23 bands per sample). Of the 16 dominant bacterial phylotypes identified, the majority were detected for the first time in MWF. Interestingly, the study revealed a crucial role for MWF brand, among other fluid factors, in modulating the community structure and dynamics.
Keywords
Exposure-levels; Risk-factors; Etiology; Diseases; Metalworking-fluids; Bacteria; Endotoxins; Metallurgical-processes; Metallurgy; Metals; Metallic-compounds; Metal-compounds; Lubricants; Fungi; Microbiology; Pathogenicity; Author Keywords: Metalworking fluid; real-time qPCR; DGGE; endotoxin
Contact
Jagjit S. Yadav, Department of Environmental Health, University of Cincinnati, College of Medicine, Cincinnati, OH 45267-0056
CODEN
FMLED7
Publication Date
20140301
Document Type
Journal Article
Email Address
jagjit.yadav@uc.edu
Funding Type
Grant
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-007364; M062014
Issue of Publication
3
ISSN
0168-6496
Source Name
FEMS Microbiology Ecology
State
OH
Performing Organization
University of Cincinnati
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