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Estimating the effective density of engineered nanomaterials for in vitro dosimetry.

Authors
DeLoid-G; Cohen-JM; Darrah-T; Derk-R; Rojanasakul-L; Pyrgiotakis-G; Wohlleben-W; Demokritou-P
Source
Nat Commun 2014 Mar; 5:3514
NIOSHTIC No.
20044300
Abstract
The need for accurate in vitro dosimetry remains a major obstacle to the development of cost-effective toxicological screening methods for engineered nanomaterials. An important key to accurate in vitro dosimetry is the characterization of sedimentation and diffusion rates of nanoparticles suspended in culture media, which largely depend upon the effective density and diameter of formed agglomerates in suspension. Here we present a rapid and inexpensive method for accurately measuring the effective density of nano-agglomerates in suspension. This novel method is based on the volume of the pellet obtained by benchtop centrifugation of nanomaterial suspensions in a packed cell volume tube, and is validated against gold-standard analytical ultracentrifugation data. This simple and cost-effective method allows nanotoxicologists to correctly model nanoparticle transport, and thus attain accurate dosimetry in cell culture systems, which will greatly advance the development of reliable and efficient methods for toxicological testing and investigation of nano-bio interactions in vitro.
Keywords
Nanotechnology; Dosimetry; In-vitro-study; Toxicology; Particulates; Cell-culture-techniques; Models; Exposure-levels
Contact
Glen DeLoid, Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Ctr Nanotechnol & Nanotoxicol, 655 Huntington Ave, Boston, MA 02115
CODEN
NCAOBW
Publication Date
20140301
Document Type
Journal Article
Email Address
gdeloid@hsph.harvard.edu
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
M052014
ISSN
2041-1723
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Nature Communications
State
MA; NC; WV
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