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Development, evaluation and comparison of two independent sampling and analytical methods for ortho-phthalaldehyde vapors and condensation aerosols in air.

Authors
Tucker-SP
Source
Anal Methods 2014 Apr; 6(8):2592-2607
NIOSHTIC No.
20044125
Abstract
Two independent sampling and analytical methods for ortho-phthalaldehyde (OPA) in air have been developed, evaluated and compared: (1) a reagent-coated solid sorbent HPLC-UV method and (2) an impinger-fluorescence method. In the first method, air sampling is conducted at 1.0 L min-1 with a sampler containing 350 mg of silica gel coated with 1 mg of acidified 2,4-dinitrophenylhydrazine (DNPH). After sampling, excess DNPH in ethyl acetate is added to the sampler prior to storage for 68 hours. The OPA-DNPH derivative is eluted with 4.0 mL of dimethyl sulfoxide (DMSO) for measurement by HPLC with a UV detector set at 385 nm. The estimated detection limit is 0.016 g per sample or 0.067 g m-3 (0.012 ppb) for a 240 L air sample. Recoveries of vapor spikes at levels of 1.2 to 6.2 g were 96 to 101%. Recoveries of spikes as mixtures of vapor and condensation aerosols were 97 to 100%. In the second method, air sampling is conducted at 1.0 L min-1 with a midget impinger containing 10 mL of DMSO solution containing N-acetyl-l-cysteine and ethylenediamine. The fluorescence reading is taken 80 min after the completion of air sampling. Since the time of taking the fluorescence reading is critical, the reading is taken with a portable fluorometer. The estimated detection limit is 0.024 g per sample or 0.1 g m-3 (0.018 ppb) for a 240 L air sample. Recoveries of OPA vapor spikes at levels of 1.4 to 5.0 g per sample were 97 to 105%. Recoveries of spikes as mixtures of vapors and condensation aerosols were 95 to 99%. The collection efficiency for a mixture of vapor and condensation aerosol was 99.4%. The two methods were compared side-by-side in a generation system constructed for producing controlled atmospheres of OPA vapor in air. Average air concentrations of OPA vapor found by both methods agreed within +/-10%.
Keywords
Sampling; Analytical-methods; Air-quality; Air-sampling; Vapors; Aerosols; Organic-solvents; Organic-compounds; Chemical-properties; Analytical-processes
Contact
Robert Streicher, CDC/NIOSH, 4676 Columbia Parkway, Cincinnati, OH 45226
CAS No.
643-79-8
Publication Date
20140421
Document Type
Journal Article
Email Address
rps3@cdc.gov
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
M042014
Issue of Publication
8
ISSN
1759-9660
NIOSH Division
DART
Priority Area
Healthcare and Social Assistance
Source Name
Analytical Methods
State
OH
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