Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Myosin immunohistochemistry and fiber adaptation following stretch-shortening contraction loading: influence of glutathione and age.

Authors
Baker-BA; Hollander-MS; Kashon-ML; Cutlip-RG
Source
Med Sci Sports Exerc 2009 May; 41(5)(Suppl 1):131
NIOSHTIC No.
20044026
Abstract
The extent and contribution of myosin heavy chain plasticity in the adaptive response following glutathione modulation and repetitive mechanical loading is not known. PURPOSE: The purpose of this research was to identify the influence dietary supplementation with a glutathione antagonist (L-Buthionine Sulfoximine (BSO)) has on the adaptability of skeletal muscle during chronic high-intensity mechanical loading via stretch-shortening contractions (SSCs) in young and old rats. METHODS: Left dorsiflexor muscles of young (12 weeks, N= 22) and old (30 months, N = 22), vehicle- (VEH) and BSO-treated rats were exposed 3 times per week for 4.5-weeks to a protocol of 80 maximal SSCs per exposure in vivo, while cage, age-matched rats served as controls (CON). Myofiber plasticity was characterized by myosin heavy chain (MHCslow+, MHCfast+, or MHCdev+) response by immunohistochemical analysis, as well as by changes in muscle cross-sectional area distribution following the exposure period. RESULTS: MHCslow+ content was increased in the old rats, irrespective of treatment or exposure. Conversely, MHCfast+ content was increased in the young rats, regardless of treatment of exposure. MHCdev+ content was not changed following chronic SSC loading. Shifts to larger fiber cross-sectional areas were apparent for all SSC-exposed groups. CONCLUSIONS: MHCdev+ is not expressed following chronic SSC exposure, regardless of age, treatment, or exposure. Aging skeletal muscle does retain some capacity for adaptation; however aging apparently diminishes the adaptive response following chronic SSC exposure. Evidently, a population of small myofibers does exist that do not undergo regeneration in aged skeletal muscle, perhaps accounting for the disparity observed between morphological adaptation and functional performance.
Keywords
Animals; Laboratory-animals; Musculoskeletal-system; Muscles; Muscle-function; Physiological-effects; Physiology; Age-groups; Exposure-levels
CODEN
MSPEDA
CAS No.
70-18-8
Publication Date
20090501
Document Type
Abstract
Email Address
bwb3@cdc.gov
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Issue of Publication
5
ISSN
0195-9131
NIOSH Division
HELD
Source Name
Medicine and Science in Sports and Exercise
State
WV
TOP