Temporal changes in rat liver gene expression after cadmium and chromium exposure.
Madejczyk-MS; Baer-CE; Dennis-WE; Minarchick-VC; Leonard-SS; Jackson-DA; Lewis-JA; Stallings-JD
Toxicologist 2014 Mar; 138(1):344
Our Nation's Guardians are at risk of exposure to a variety of environmental health hazards throughout their normal duty activities including deployments, training exercises, and homeland defense situations. Metals are widely used in large quantities in a number of industrial processes and are common environmental toxicants, which increases the possibility of our Soldiers being exposed at toxic levels. While metal toxicity has been widely studied, the exact mechanisms of toxicity remain unclear. In order to further elucidate these mechanisms and identify candidate biomarkers, rats were exposed via a single intraperitoneal injection to three concentrations of CdCl2, NiCl2 and Na2Cr2O7, and livers were harvested 1, 3, or 7 days after exposure. Cd and Cr accumulated in the liver at 1 day post-exposure, while there was no accumulation of Ni. Cd levels remained elevated over the length of the experiment, while Cr levels declined. Differentially expressed genes were identified via microarray analysis. Both common and uniquely modulated transcripts and perturbed pathways were identified for the metal species. Enriched pathways and functions included hepatic injury, inflammation, cell cycle, tissue repair, and cancer. This work provides insight into the temporal effects and mechanistic pathways involved in acute metal intoxication, which may lead to the identification of candidate biomarkers.
Toxicology; Exposure-levels; Chemical-composition; Workers; Work-environment; Humans; Men; Women; Environmental-exposure; Environmental-hazards; Environmental-health; Metal-compounds; Metallic-compounds; Toxins; Soldiers; Biomarkers; Animals; Laboratory-animals
7440-43-9; 7440-47-3; 7440-02-0; 10108-64-2; 10588-01-9; 7718-54-9
The Toxicologist. Society of Toxicology 53rd Annual Meeting and ToxExpo, March 23-27, 2014, Phonex, Arizona