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Efficacy of screens in removing long fibers from an aerosol stream -- sample preparation technique for toxicology studies.

Authors
Ku-BK; Deye-GJ; Turkevich-LA
Source
Inhal Toxicol 2014 Feb; 26(2):70-83
NIOSHTIC No.
20043663
Abstract
Fiber dimension (especially length) and biopersistence are thought to be important variables in determining the pathogenicity of asbestos and other elongate mineral particles. In order to prepare samples of fibers for toxicology studies, it is necessary to develop and evaluate methods for separating fibers by length in the micrometer size range. In this study, we have filtered an aerosol of fibers through nylon screens to investigate whether such screens can efficiently remove the long fibers (L >20 microm, a typical macrophage size) from the aerosol stream. Such a sample, deficient in long fibers, could then be used as the control in a toxicology study to investigate the role of length. A well-dispersed aerosol of glass fibers (a surrogate for asbestos) was generated by vortex shaking a Japan Fibrous Material Research Association (JFMRA) glass fiber powder. Fibers were collected on a mixed cellulose ester (MCE) filter, imaged with phase contrast microscopy (PCM) and lengths were measured. Length distributions of the fibers that penetrated through various screens (10, 20 and 60 microm mesh sizes) were analyzed; additional study was made of fibers that penetrated through double screen and centrally blocked screen configurations. Single screens were not particularly efficient in removing the long fibers; however, the alternative configurations, especially the centrally blocked screen configuration, yielded samples substantially free of the long fibers.
Keywords
Fibrous-bodies; Inhalants; Particulates; Pathogenicity; Sample-preparation; Toxic-materials; Filters; Filter-materials; Aerosol-particles; Aerosol-sampling; Fibrous-glass; Microscopic-analysis; Analytical-instruments; Analytical-processes; Author Keywords: Aerosol; fiber length; glass fiber; nylon mesh screens; vortex shaker
Contact
Bon Ki Ku, Division of Applied Research and Technology, Centers for Disease Control and Prevention (CDC), National Institute for Occupational Safety and Health (NIOSH), 4676 Columbia Parkway, MS-R3, Cincinnati, OH 45226, USA
CODEN
INHTE5
Publication Date
20140201
Document Type
Journal Article
Email Address
bku@cdc.gov
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
M012014
Issue of Publication
2
ISSN
0895-8378
NIOSH Division
DART
Priority Area
Manufacturing; Mining
Source Name
Inhalation Toxicology
State
OH
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