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Asymmetric dimethylarginine blocks nitric oxide-mediated alcohol-stimulated cilia beating.

Authors
Wyatt-TA; Wells-SM; Alsaidi-ZA; DeVasure-JM; Klein-EB; Bailey-KL; Sisson-JH
Source
Mediators Inflamm 2013 Nov; 2013:592892
NIOSHTIC No.
20043636
Abstract
The airway epithelium is exposed to alcohol during drinking through direct exhalation of volatized ethanol from the bronchial circulation. Alcohol exposure leads to a rapid increase in the cilia beat frequency (CBF) of bronchial epithelial cells followed by a chronic desensitization of cilia stimulatory responses. This effect is governed in part by the nitric oxide regulation of cyclic guanosine and adenosine monophosphate-dependent protein kinases (PKG and PKA) and is not fully understood. Asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, is implicated in the pathogenesis of several pulmonary disorders. We hypothesized that the inhibition of nitric oxide synthase by ADMA blocks alcohol-stimulated increases in CBF. To test this hypothesis, ciliated primary bovine bronchial epithelial cells (BBEC) were preincubated with ADMA (100 ÁM) and stimulated with 100 mM ethanol. CBF was measured and PKA assayed. By 1 hr, ethanol activated PKA, resulting in elevated CBF. Both alcohol-induced PKA activation and CBF were inhibited in the presence of ADMA. ADMA alone had no effect on PKA activity or CBF. Using a mouse model overexpressing the ADMA-degrading enzyme, dimethylarginine dimethylaminohydrolase (DDAH), we examined PKA and CBF in precision-cut mouse lung slices. Alcohol-stimulated increases in lung slice PKA and CBF were temporally enhanced in the DDAH mice versus control mice.
Keywords
Pulmonary-system; Alcoholic-beverages; Alcohols; Cellular-reactions; Cell-alteration; Stimulants; Proteins; Nitrogen-oxides; Oxides; Pathogenesis; Laboratory-testing; Cell-cultures; Bioassays; Laboratory-animals; Lung-cells; Lung-irritants; Alcoholism; Immunological-tests; Lung-function
Contact
T. A. Wyatt, VA Nebraska-Western Iowa Health Care System Research Service, Department of Veterans Affairs Medical Center, 4101 Woolworth Avenue, Omaha, NE 68105, USA
CODEN
MNFLEF
CAS No.
64-17-5; 10102-43-9
Publication Date
20131106
Document Type
Journal Article
Funding Type
Cooperative Agreement
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U54-OH-010162
ISSN
0962-9351
Source Name
Mediators of Inflammation
State
NE
Performing Organization
University of Nebraska
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