Correlation of chromosome damage and promoter methylation status of the DNA repair genes MGMT and hMLH1 in Chinese vinyl chloride monomer (VCM)-exposed workers.
Wu-F; Liu-J; Qiu-Y-L; Wang-W; Zhu-S-M; Sun-P; Miao-W-B; Li-Y-L; Brandt-Rauf-PW; Xia-Z-L
Int J Occup Med Environ Health 2013 Mar; 26(1):173-182
OBJECTIVE: To explore the association of the methylation status of MGMT and hMLH1 with chromosome damage induced by vinyl chloride monomer (VCM). MATERIALS AND METHODS: Methylation of MGMT and hMLH1 was measured in 101 VCM-exposed workers by methylation-specific PCR. Chromosome damage in peripheral blood lymphocytes was measured by the cytokinesis-block micronucleus assay. The subjects were divided into chromosome damaged and non-damaged groups based on the normal reference value of micronuclei frequencies determined for two control groups. RESULTS: MGMT promoter methylation was detectable in 5 out of 49 chromosome damaged subjects, but not in the chromosome non-damaged subjects; there was a significant difference in MGMT methylation between the two groups (p < 0.05). CONCLUSIONS: We detected aberrant promoter methylation of MGMT in a small number of chromosome damaged VCM-exposed workers, but not in the chromosome non-damaged subjects. This preliminary observation warrants further investigation in a larger study.
DNA-damage; Chromosome-damage; Genetics; Metabolism; Drug-interaction; Enzymes; Proteins; Monomers; Bioassays; Blood-cells; Lymphocytes; Gases; Ethylenes; Chlorides; Genes; Polymerization; Humans; Men; Women; Tumor-inhibition; Exposure-assessment;
Author Keywords: Vinyl chloride monomer; Chromosome damage; MGMT; hMLH1; DNA methylation
Z-L Xia, Department of Occupational Health and Toxicology, School of Public Health, Fudan University, 138 Yixueyuan Road, Shanghai 200032, China
International Journal of Occupational Medicine and Environmental Health
University of Illinois