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Indirect homologous competitive enzyme-linked immunosorbent assay for the detection of a class of glycosylated dihydrochalcones.

Authors
Ranganathan-A; Paradise-GA; Hansen-CA; McCoy-MR; Gee-SJ; Zhong-P; Chang-D; Hammock-BD
Source
J Agric Food Chem 2013 Jul; 61(28):6964-6970
NIOSHTIC No.
20043567
Abstract
Hesperetin dihydrochalcone 4'-glucoside, 1, and phloretin 4'-glucoside, 2, belong to a family of dihydrochalcone glycosides that exhibit flavorant properties. In this study was developed a competitive, indirect homologous ELISA for the detection of targets 1 and 2 in fermentation media. Immunogen and coating antigen were prepared by conjugating hapten, 4-(3-oxo-3-(2,6-dihydroxy-4-glucoside phenyl)propyl) benzoic acid, to thyroglobulin and bovine serum albumin, respectively. Antibodies raised in rabbits M6122, M6123, and M6124 and the coating antigen were screened and characterized to determine their optimum concentrations. The optimized ELISA, developed with antibody M6122, gave IC50 values of 27.8 and 21.8 ng/mL for 1 and 2, respectively. Selectivity of the assay was assessed by measuring cross-reactivity of antibody M6122 to related congeners such as aglycones and the 2'-glycosides of hesperetin dihydrochalcone, 5 and phloretin, 6. Antibody M6122 showed very low recognition of 5 and virtually no recognition of the aglycones and 6.
Keywords
Animals; Laboratory-animals; Immunology; Antibody-response; Analytical-processes; Chemical-properties; Biosynthesis; Author Keywords: dihydrochalcone glycoside; microbial fermentation; hapten; polyclonal antibodies; ELISA
Contact
Bruce D. Hammock, Department of Entomology and UCD Comprehensive Cancer Center, University of California Davis, Davis, California 95616
CODEN
JAFCAU
Publication Date
20130701
Document Type
Journal Article
Email Address
bdhammock@ucdavis.edu
Funding Type
Cooperative Agreement; Agriculture
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007550
Issue of Publication
28
ISSN
0021-8561
Source Name
Journal of Agricultural and Food Chemistry
State
CA; OH
Performing Organization
University of California - Davis
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