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IL-6 deficiency exacerbates skin inflammation in a murine model of irritant dermatitis.

Authors
Lee-EG; Mickle-Kawar-BM; Gallucci-RM
Source
J Immunotoxicol 2013 Apr-Jun; 10(2):192-200
NIOSHTIC No.
20043542
Abstract
Contact dermatitis is the second most reported occupational injury associated with workers compensation. Inflammatory cytokines are closely involved with the development of dermatitis, and their modulation could exacerbate skin damage, thus contributing to increased irritancy. IL-6 is a pro-inflammatory cytokine paradoxically associated with both skin healing and inflammation. To determine what role this pleiotropic cytokine plays in chemically-induced irritant dermatitis, IL-6 deficient (KO), IL-6 over-expressing transgenic (TgIL6), and corresponding wild-type (WT) mice were exposed to acetone or the irritants JP-8 jet fuel or benzalkonium chloride (BKC) daily for 7 days. Histological analysis of exposed skin was performed, as was tissue mRNA and protein expression patterns of inflammatory cytokines via QPCR and multiplex ELISA. The results indicated that, following JP-8 exposure, IL-6KO mice had greatly increased skin IL-1, TNFa, CCL2, CCL3, and CXCL1 mRNA and corresponding product protein expression when compared to that of samples from WT counterparts and acetone-exposed control mice. BKC treatment induced the expression of all cytokines examined as compared to acetone, with CCL2 significantly higher in skin from IL-6KO mice. Histological analysis showed that IL-6KO mice displayed significantly more inflammatory cell infiltration as compared to WT and TgIL6 mice in response to jet fuel. Analysis of mRNA for the M2 macrophage marker CD206 indicated a 4-fold decrease in skin of IL-6KO mice treated with either irritant as compared to WT. Taken together, these observations suggest that IL-6 acts in an anti-inflammatory manner during irritant dermatitis, and these effects are dependent on the chemical nature of the irritant.
Keywords
Dermatitis; Skin-disorders; Skin; Injuries; Workers; Irritants; Animals; Laboratory-animals; Acetones; Jet-engine-fuels; Humans; Men; Women; Metabolism; Genetics; Hydrocarbons; Author Keywords: IL-6; dermatitis; cytokines; inflammation
Contact
Randle M. Gallucci, PhD, OUHSC College of Pharmacy, PO Box 26901, Oklahoma City, OK 73126-0901
CODEN
JIOMAP
CAS No.
67-64-1; 8001-54-5
Publication Date
20130401
Document Type
Journal Article
Email Address
randy-gallucci@ouhsc.edu
Funding Type
Grant
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R03-OH-009662; M122013
Issue of Publication
2
ISSN
1547-691X
Source Name
Journal of Immunotoxicology
State
OK
Performing Organization
University of Oklahoma Health Sciences Center
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