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Expression of non-structural-1A binding protein in lung epithelial cells is modulated by miRNA-548an on exposure to influenza A virus.

Authors
Othumpangat-S; Noti-JD; Blachere-FM; Beezhold-DH
Source
Virology 2013 Dec; 447(1-2):84-94
NIOSHTIC No.
20043412
Abstract
Understanding the host response to influenza A virus infection is essential for developing intervention approaches. We show that infection of human alveolar epithelial cells and human bronchial epithelial cells with influenza A for 3h resulted in down-regulation of host hsa-miRNA-548an (miRNA-548an) which triggered the overexpression of influenza non-structural-1A binding protein (IVNS1ABP, herein referred to as NS1ABP). Reduced NS1ABP mRNA and NS1ABP protein expression after transfection of miRNA-548an mimic or increased NS1ABP mRNA and NS1ABP protein expression after transfection of miRNA-548an inhibitor provided evidence that miRNA-548an is involved in the regulation of NS1ABP. Transfection of cells with inhibitor led to reduced apoptosis of infected cells while transfection of mimic led to increased apoptosis and reduced influenza copy number suggesting that NS1ABP has a role in viral maintenance. Thus, miRNA-548an may be an important target in controlling the early stage infection of influenza A.
Keywords
Viral-diseases; Viral-infections; Infectious-diseases; Lung-cells; Alveolar-cells; Host-resistance; Cell-alteration; Cellular-reactions; Cell-transformation; Proteins; Chemical-binding; Cell-damage; Immune-reaction; Respiratory-infections; Author Keywords: miRNA; NS1A binding protein; Influenza virus; Lung epithelial cells; Apoptosis
Contact
John D.Noti, National Institute for Occupational Safety and Health, 1095 Willowdale Road, MS 4020, Morgantown, WV 26505, USA
CODEN
VIRLAX
Publication Date
20131201
Document Type
Journal Article
Email Address
ivr2@cdc.gov
Fiscal Year
2014
NTIS Accession No.
NTIS Price
Identifying No.
M122013
Issue of Publication
1-2
ISSN
0042-6822
NIOSH Division
HELD
Priority Area
Healthcare and Social Assistance
Source Name
Virology
State
WV
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