Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Investigation of the pulmonary bioactivity of double-walled carbon nanotubes.

Authors
Sager-TM; Wolfarth-MW; Battelli-LA; Leonard-SS; Andrew-M; Steinbach-T; Endo-M; Tsuruoka-S; Porter-DW; Castranova-V
Source
J Toxicol Environ Health, A 2013 Aug; 76(15):922-936
NIOSHTIC No.
20043312
Abstract
Double-walled carbon nanotubes (DWCNT) are a rather new and unexplored variety of carbon nanotubes. Previously conducted studies established that exposure to a variety of carbon nanotubes produced lung inflammation and fibrosis in mice after pharyngeal aspiration. However, the bioactivity of double-walled carbon nanotubes (DWCNT) has not been determined. In this study, the hypothesis that DWCNT would induce pulmonary toxicity was explored by analyzing the pulmonary bioactivity of DWCNT. To test this hypothesis, C57Bl/6 mice were exposed to DWCNT by pharyngeal aspiration.Mice underwent whole-lung lavage (WLL) to assess pulmonary inflammation and injury, and lung tissue was examined histologically for development of pulmonary disease as a function of dose and time. The results showed that DWCNT exposure produced a dose-dependent increase in WLL polymorphonuclear leukocytes (PMN), indicating that DWCNT exposure initiated pulmonary inflammation. DWCNT exposure also produced a dose-dependent rise in lactate dehydrogenase (LDH) activity, as well as albumin levels, in WLL fluid, indicating that DWCNT exposure promoted cytotoxicity as well as decreases in the integrity of the blood-gas barrier in the lung, respectively. In addition, at 7 and 56 d postexposure, the presence of significant alveolitis and fibrosis was noted in mice exposed to 40 ug/mouse DWCNT. In conclusion, this study provides insight into previously uninvestigated pulmonary bioactivity of DWCNT exposure. Data indicate that DWCNT exposure promotes inflammation, injury, and fibrosis in the lung.
Keywords
Exposure-levels; Risk-factors; Lung-function; Lung-disorders; Lung; Lung-tissue; Animals; Laboratory-animals; Pulmonary-function; Pulmonary-system; Pulmonary-system-disorders; Fibrosis; Cytotoxicity
Contact
Tina M. Sager, PhD, National Institute for Occupational Safety and Health, 1095 Willowdale Road, M/S 2015, Morgantown, WV 26505
CODEN
JTEHD6
Publication Date
20130801
Document Type
Journal Article
Email Address
sst2@cdc.gov
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
M112013
Issue of Publication
15
ISSN
1528-7394
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV; VA
TOP