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Biodegradation of single-walled carbon nanotubes by eosinophil peroxidase.

Authors
Andon-FT; Kapralov-AA; Yanamala-N; Feng-W; Baygan-A; Chambers-BJ; Hultenby-K; Ye-F; Toprak-MS; Brandner-BD; Fornara-A; Klein-Seetharaman-J; Kotchey-GP; Star-A; Shvedova-AA; Fadeel-B; Kagan-VE
Source
Small 2013 Aug; 9(16):2721-2729
NIOSHTIC No.
20043135
Abstract
Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H2O 2 is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H2O2 alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials. Human eosinophil peroxidase (EPO) is able to degrade SWCNTs in vitro in the presence of H2O2. EPO is one of the major oxidant-generating enzymes present in human lungs during inflammatory states. The biodegradation of SWCNTs is evidenced also in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. These results are relevant to potential respiratory exposure to carbon nanotubes.
Keywords
Nanotechnology; Humans; Men; Women; Enzymes; Lung; Oxidative-enzymes; Models; Author Keywords: carbon nanotubes; eosinophil peroxidase; molecular modeling; biodegradable materials
Contact
Fadeel Bengt, Division of Molecular Toxicology, Institute of Environmental Medicine, Karolinska Institutet, Nobels Väg 13, Stockholm, 17177, Sweden
CODEN
SMALBC
Publication Date
20130826
Document Type
Journal Article
Email Address
bengt.fadeel@ki.se
Funding Type
Grant
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282; M102013
Issue of Publication
16
ISSN
1613-6810
NIOSH Division
HELD
Priority Area
Mining
Source Name
Small
State
PA; WV
Performing Organization
University of Pittsburgh at Pittsburgh
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