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Chronic exposure to carbon nanotubes induces invasion of human mesothelial cells through matrix metalloproteinase-2.

Authors
Lohcharoenkal-W; Wang-L; Stueckle-TA; Dinu-CZ; Castranova-V; Liu-Y; Rojanasakul-Y
Source
ACS Nano 2013 Sep; 7(9):7711-7723
NIOSHTIC No.
20043087
Abstract
Malignant mesothelioma is one of the most aggressive forms of cancer known. Recent studies have shown that carbon nanotubes (CNTs) are biopersistent and induce mesothelioma in animals, but the underlying mechanisms are not known. Here, we investigate the effect of long-term exposure to high aspect ratio CNTs on the aggressive behaviors of human pleural mesothelial cells, the primary cellular target of human lung mesothelioma. We show that chronic exposure (4 months) to single- and multiwalled CNTs induced proliferation, migration, and invasion of the cells similar to that observed in asbestos-exposed cells. An up-regulation of several key genes known to be important in cell invasion, notably matrix metalloproteinase-2 (MMP-2), was observed in the exposed mesothelial cells as determined by real-time PCR. Western blot and enzyme activity assays confirmed the increased expression and activity of MMP-2. Whole genome microarray analysis further indicated the importance of MMP-2 in the invasion gene signaling network of the exposed cells. Knockdown of MMP-2 in CNT and asbestos-exposed cells by shRNA-mediated gene silencing effectively inhibited the aggressive phenotypes. This study demonstrates CNT-induced cell invasion and indicates the role of MMP-2 in the process.
Keywords
Nanotechnology; Respiratory-system-disorders; Mesothelial-cells; Metalloproteins; Cancer; Malignancy; Long-term-exposure; Lung-cancer; Lung-cells; Chronic-exposure; Cell-alteration; Cell-damage; Cellular-reactions; Cell-migration; Bioassays; Enzyme-activity; Genes; Genotoxic-effects; Author Keywords: carbon nanotubes; asbestos; mesothelioma; MMP-2; mesothelial cells; cell invasion
Contact
Yon Rojanasakul, Department of Pharmaceutical Sciences and Mary Babb Randolph Cancer Center, West Virginia University, Morgantown, WV 26506
CODEN
ANCAC3
CAS No.
7440-44-0
Publication Date
20130901
Document Type
Journal Article
Email Address
yrojan@hsc.wvu.edu
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
M082013
Issue of Publication
9
ISSN
1936-0851
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
ACS Nano
State
WV
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