Laboratory studies of the impact of calcite on in vitro and in vivo effects of coal dust: a potential preventive agent for coal workers' pneumoconiosis?
Aladdin-M; Jian-J; Yang-Q; Chen-L-C; Finkelman-RB; Huang-X
Am J Ind Med 2013 Mar; 56(3):292-299
Background: Bioavailable iron (BAI) in coal, which may play a key role in causing coal workers' pneumoconiosis (CWP), is present at relatively high levels in Appalachian coals. Calcite decreases BAI and is more plentiful in Western coals than in Appalachian coals, possibly explaining the lower CWP prevalence among Western miners. Methods: We measured effects of calcite on BAI in non-cellular and cellular systems involving Pennsylvania (PA) coal dust. We also tested in vivo effects of calcite on transferrin receptor and markers of epithelial mesenchymal transition (EMT) and inflammation in mice exposed to PA coal. Results: Calcite rapidly eliminated BAI in an aqueous suspension of PA coal. Ferritin induction in human lung epithelial cells exposed to PA coal was effectively eliminated by calcite. Mouse lung tissue markers indicated increased EMT after exposure to PA coal dust, but not after exposure to PA coal plus calcite. Markers of inflammation increased following exposure to PA coal alone, but not following exposure to PA coal plus calcite. Conclusion: Additional research may lead to the use of supplemental calcite in coal mining as a safe and effective way to prevent CWP among Appalachian coal miners.
Laboratory-testing; In-vitro-study; In-vivo-study; Disease-prevention; Coal-workers-pneumoconiosis; Coal-dust; Coal-miners; Mining-industry; Coal-mining; Respiratory-system-disorders; Pulmonary-system-disorders; Lung-disease; Lung-fibrosis; Iron-compounds; Calcium-compounds; Laboratory-animals; Immune-reaction; Lung-cells; Biomarkers;
Author Keywords: iron; calcium; coal workers' pneumoconiosis (CWP); epithelial mesenchymal transition (EMT); fibrosis
Xi Huang, PhD, New York University School of Medicine, Hospital for Joint Diseases, Room1600, 550 First Avenue, NewYork, NY 10016
471-34-1; 7440-70-2; 7439-89-6
Grant-Number-R01-OH-009771; B20130805; M082013
American Journal of Industrial Medicine
New York University School of Medicine - New York