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Characterization of Cannabis sativa allergens.

Authors
Nayak-AP; Green-BJ; Sussman-G; Berlin-N; Lata-H; Chandra-S; ElSohly-MA; Hettick-JM; Beezhold-DH
Source
Ann Allergy, Asthma, & Immun 2013 Jul; 111(1):32-37.e4
NIOSHTIC No.
20042914
Abstract
Background: Allergic sensitization to Cannabis sativa is rarely reported, but the increasing consumption of marijuana has resulted in an increase in the number of individuals who become sensitized. To date, little is known about the causal allergens associated with C sativa. Objective: To characterize marijuana allergens in different components of the C sativa plant using serum IgE from marijuana sensitized patients. Methods: Serum samples from 23 patients with a positive skin prick test result to a crude C sativa extract were evaluated. IgE reactivity was variable between patients and C sativa extracts. IgE reactivity to C sativa proteins in Western blots was heterogeneous and ranged from 10 to 70 kDa. Putative allergens derived from 2-dimensional gels were identified. Results: Prominent IgE reactive bands included a 23-kDa oxygen-evolving enhancer protein 2 and a 50-kDa protein identified to be the photosynthetic enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase. Additional proteins were identified in the proteomic analysis, including those from adenosine triphosphate synthase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and luminal binding protein (heat shock protein 70), suggesting these proteins are potential allergens. Deglycosylation studies helped refine protein allergen identification and demonstrated significant IgE antibodies against plant oligosaccharides that could help explain cross-reactivity. Conclusion: Identification and characterization of allergens from C sativa may be helpful in further understanding allergic sensitization to this plant species.
Keywords
Drug-abuse; Drug-interaction; Drugs; Allergens; Allergic-reactions; Allergies; Sensitization; Psychotropic-drugs; Immune-reaction; Immunological-tests; Plant-substances; Hypersensitivity; Respiratory-system-disorders; Humans; Skin-tests; Sampling; Proteins; Enzyme-activity; Enzymes; Antibody-response
Contact
Donald H. Beezhold, PhD, Allergy and Clinical Immunology Branch, 1095 Willowdale Rd, National Institute for Occupational Safety and Health, Morgantown, WV 26505
CODEN
ALAIF6
Publication Date
20130701
Document Type
Journal Article
Email Address
zec1@cdc.gov
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
B20130805
Issue of Publication
1
ISSN
1081-1206
NIOSH Division
HELD
Priority Area
Healthcare and Social Assistance; Services
Source Name
Annals of Allergy, Asthma, and Immunology
State
WV; MS
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