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Nerve growth factor reduces amiloride-sensitive Na+ transport in human airway epithelial cells.

Authors
Shimko-MJ; Zaccone-EJ; Thompson-JA; Schwegler-Berry-D; Willard-PA; Fedan-JS
Source
FASEB J 2013 Apr; 27(Meeting Abstracts):1107.15
NIOSHTIC No.
20042864
Abstract
Nerve growth factor (NGF) is over-expressed in patients with lung inflammatory diseases including airway virus infections. Optimum airway surface liquid (ASL), controlled by epithelial cells, is essential for normal lung function. NGF has been shown to affect ion transport properties of neurons, but no information is available as to the effect NGF has on ion transport of airway epithelial cells. To investigate if NGF can alter epithelial ion transport, we established human primary cultured epithelial cells in air-interface that demonstrates desirable in situ morphology and ion transport function. These cells were placed in an Ussing chamber to obtain transepithelial voltage (Vt), short-circuit current (Isc), transepithelial resistance (Rt), and to measure responses to known ion transport inhibitors. Cells had an average Vt of -7.1 mV, Isc of 5.9 µA, and Rt of 750 µxcm2. Amiloride (apical, 3.5x10-5 M) decreased total /sc by 52%. When cells were first treated with 1 ng/ml NGF prior to the addition of amiloride, amiloride reduced total /sc by 38%. The addition of NGF (apical, 1 ng/ml) to the bath reduced the total Isc by 4.5 %. These results indicate that NGF reduces Na+ transport in airway epithelial cells. This could result in an increase in ASL height. Support provided by NIOSH.
Keywords
Nerve-function; Nerve-tissue; Nerves; Lung; Lung-disease; Lung-disorders; Viral-diseases; Diseases; Humans; Men; Women
CODEN
FAJOEC
Publication Date
20130401
Document Type
Abstract
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
B20130801
ISSN
0892-6638
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
The FASEB Journal
State
WV
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