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Mercury biomarkers and DNA methylation among Michigan dental professionals.

Authors
Goodrich-JM; Basu-N; Franzblau-A; Dolinoy-DC
Source
Environ Mol Mutagen 2013 Apr; 54(3):195-203
NIOSHTIC No.
20042586
Abstract
Modification of the epigenome may be a mechanism underlying toxicity and disease following chemical exposure. Animal and human data suggest that mercury (Hg) impacts DNA methylation. We hypothesize that methylmercury and inorganic Hg exposures from fish consumption and dental amalgams, respectively, may be associated with altered DNA methylation at global repetitive elements (long interspersed elements, LINE-1) and candidate genes related to epigenetic processes (DNMT1) and protection against Hg toxicity (SEPW1, SEPP1). Dental professionals were recruited at Michigan Dental Association (MDA) meetings in 2009 and 2010. Subjects (n=131) provided survey data (e.g. exposure sources, demographics) and biological samples for Hg measurement and epigenetic analysis. Total Hg was quantified via atomic absorption spectrophotometry in hair and urine, indicative of methylmercury and inorganic Hg exposures, respectively. Global repetitive and candidate gene methylation was quantified via pyrosequencing of bisulfite converted DNA isolated from buccal mucosa. Hair Hg (geometric mean (95% CI): 0.37 (0.31-0.44) g/g) and urine Hg (0.70 (0.60-0.83) g/L) were associated with sources of exposure (fish consumption and dental amalgams, respectively). Multivariable linear regression revealed a trend of SEPP1 hypomethylation with increasing hair Hg levels, and this was significant (P<0.05) among males. The trend remained when excluding non-dentists. No significant relationships between urine Hg and DNA methylation were observed. Thus, in a limited cohort, we identified an association between methylmercury exposure and hypomethylation of a potentially labile region of the genome (SEPP1 promoter), and this relationship was gender specific.
Keywords
Dental-materials; Dentists; Mercury-compounds; Employee-exposure; Environmental-exposure; Humans; Biomarkers; Genes; Methyl-compounds; Urinalysis; Analytical-processes; Genetic-factors; Genotoxic-effects; DNA-damage; Food; Inorganic-mercury; Professional-workers; Atomic-absorption-spectrometry; Men; Women; Chemical-analysis; Sampling; Proteins; Nucleotides; Sex-factors; Sulfites; Author Keywords: epigenetics; environmental exposure; occupational exposure; selenoproteins
Contact
Jaclyn M. Goodrich, 1415 Washington Heights, Ann Arbor, MI 48109, USA
CODEN
EMMUEG
CAS No.
7439-97-6; 22967-92-6
Publication Date
20130401
Document Type
Journal Article
Email Address
gaydojac@umich.edu
Funding Type
Grant
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-T42-OH-008455; B20130531
Issue of Publication
3
ISSN
0893-6692
Source Name
Environmental and Molecular Mutagenesis
State
MI
Performing Organization
University of Michigan, Ann Arbor
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