Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Interlaboratory evaluation of rodent pulmonary responses to engineered nanomaterials: the NIEHS Nano Go Consortium.

Authors
Bonner-JC; Silva-RM; Taylor-AJ; Brown-JM; Hilderbrand-SC; Castranova-V; Porter-D; Elder-A; Oberdörster-G; Harkema-JR; Bramble-LA; Kavanagh-TJ; Botta-D; Nel-A; Pinkerton-KE
Source
Environ Health Perspect 2013 Jun; 121(6):676-682
NIOSHTIC No.
20042555
Abstract
Background: Engineered nanomaterials (ENMs) have potential benefits, but also present safety concerns for human health. Inter-laboratory studies in rodents using standardized protocols are needed for ENM toxicity assessment. Methods: Four labs evaluated lung responses in C57BL/6 mice to ENMs delivered by oropharyngeal aspiration (OPA). Three labs evaluated Sprague-Dawley (SD) or Fisher (F)344 rats following intratracheal instillation (IT). ENMs tested were three forms of titanium dioxide (TiO2); anatase/rutile spheres (TiO2-P25), anatase spheres (TiO2-A), anatase nanobelts (TiO2-NB), and three forms of multiwalled carbon nanotubes (MWCNT); original (O), purified (P), and carboxylic acid "functionalized" (F). Bronchoalveolar lavage fluid was collected after 1 day for differential cell counts, lactate dehydrogenase (LDH), and protein. Lungs were fixed for histopathology. Responses were also examined at 7 days (TiO2) and 21 days (MWCNTs). Results: TiO2-A, TiO2-P25, and TiO2-NB caused significant neutrophilia in mice at 1 day in 3 out of 4 labs, respectively. TiO2-NB caused neutrophilia in rats at 1 day in 2 out of 3 labs, while TiO2-P25 or TiO2-A had no significant effect in any of the labs. Inflammation induced by TiO2 in mice and rats resolved by day 7. All MWCNT types caused neutrophilia at 1 day in 3 out of 4 mouse labs and all rat labs. Three out of 4 labs observed similar histopathology to O-MWCNT or TiO2-NB in mice. Conclusions: ENMs produced similar patterns of neutrophilia and pathology in rats and mice. Although inter-laboratory variability was found in the degree of neutrophilia caused by the three types of TiO2 nanoparticles, similar findings of relative potency for the three types of MWCNTs were found across all laboratories, thus providing greater confidence in these inter-laboratory comparisons.
Keywords
Nanotechnology; Animal-studies; Laboratory-animals; Laboratory-testing; Respiratory-system-disorders; Pulmonary-system-disorders; Lung-disorders; Lung-function; Carboxylic-acids; Alveolar-cells; Proteins; Cellular-reactions; Histopathology; Neutrophils; Immune-reaction; Dose-response; Dioxides; Author Keywords: carbon nanotubes; inflammation; lung; nanoparticles; titanium dioxide
Contact
James C. Bonner, Department of Environmental and Molecular Toxicology, North Carolina State University, Raleigh, NC 27606 USA
CODEN
EVHPAZ
CAS No.
13463-67-7; 7440-44-0
Publication Date
20130601
Document Type
Journal Article
Email Address
james_bonner@ncsu.edu
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
B20130520
Issue of Publication
6
ISSN
0091-6765
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Environmental Health Perspectives
State
NC; CA; WV; NY; MI; WA
TOP