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Cytotoxic effects of dicyclohexylamine and three metalworking fluids on human epidermal keratinocytes.

Authors
Linthicum-AD; Inman-AO; Monteiro-Riviere-NA; Baynes-RE
Source
Toxicologist 2013 Mar; 132(1):222
NIOSHTIC No.
20042406
Abstract
Many of the 1.2 million workers in the metal machining industry within the United States will be exposed to metalworking fluids via the dermal route, making it a major occupational health and safety concern. Dicyclohexylamine (DCHA), an anticorrosive agent used in the metalworking industry to prevent corrosion of fabricated materials, is known to permeate the skin and could potentially elicit a toxic response on human epidermal keratinocytes (HEK). HEK were exposed only to DCHA or one of the following three generic metalworking fluids: soluble oil, synthetic oil and semi-synthetic oil for 24h. Cells were dosed with solutions ranging from concentrations of 200-5000 microg/ml. The highest concentration of 5000 microg/ml is the relevant occupational exposure dose in the metal machining industry. Cells exposed to 200-5000 microg/ml of DCHA resulted in a 24-91% decrease in cell viability in comparison to the media control. The soluble oil and semi-synthetic oil dosed from 200-5000 microg/ml resulted in an 80-83% and 81-85% decrease in cell viability, respectively, in comparison to the media control. For synthetic oil, there was a 52-85% decrease in cell viability in comparison to the media control. DCHA and all metalworking fluid treatments caused a significant (p<0.05) decrease in HEK viability. In conclusion, metalworking fluids and DCHA had a cytotoxic effect to HEK. This data suggests that the concentration of DCHA in metalworking fluid formulations should be reduced while safer metalworking fluid formulations are developed.
Keywords
Toxicology; Laboratory-techniques; Exposure-levels; Dose-response; Immune-reaction; Cell-function; Cellular-reactions; Cytotoxic-effects; Metal-industry; Metal-industry-workers; Metal-workers; Metalworking; Metalworking-fluids; Metalworking-industry; Skin-exposure; Skin-irritants; Skin-sensitivity; Cyclohexylamines; Allergic-dermatitis; Corrosion-inhibitors; Corrosion-resistant-coatings; Skin-absorption; Synthetics; Oil-dermatitis; Oils; Machine-operators; Cell-damage; Cell-cultures; Fluids; Employee-exposure
CAS No.
101-83-7
Publication Date
20130301
Document Type
Abstract
Funding Amount
875919
Funding Type
Grant
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003669; B20130416
Issue of Publication
1
ISSN
1096-6080
Priority Area
Disease and Injury: Allergic and Irritant Dermatitis
Source Name
The Toxicologist. Society of Toxicology 52nd Annual Meeting and ToxExpo, March 10-14, 2013, San Antonio, Texas
State
NC; KS; TX
Performing Organization
North Carolina State University, Raleigh, North Carolina
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