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1-Hydroxypyrene as a biomarker for assessing the effects of exposure to polycyclic aromatic hydrocarbons on semen quality and sperm DNA integrity.

Authors
Jeng-HA; Pan-C-H; Chao-M-R
Source
J Environ Sci Health. Part A, Tox Hazard Subst Environ Eng 2013 Jan; 48(2):152-158
NIOSHTIC No.
20042304
Abstract
A cross-sectional study was conducted to assess whether urinary 1-hydroxypyrene (1-OHP) could serve as a biomarker to assess the effect of PAHs on cellular and molecular changes of sperm. Urine and semen samples were collected from a total of 65 healthy coke oven workers. Sperm quality parameters (concentration, motility, vitality, and morphology) and semen integrity (DNA fragmentation, 8-oxodGuo, bulky DNA adducts) were analyzed. Sixteen (16) targeted PAHs at the personal breathing zone area were monitored and quantified. Results showed that urinary 1-OHP positively correlated with measured levels of 16 targeted PAHs. Urinary 1-OHP did not significantly correlate with semen quality; however, PAHs with heavy molecular weight, e.g., benzo(g,h,i)perylene and benzo(k)fluoranthene, negatively correlated with morphology and motility of sperms (p = 0.02 and 0.002, p = 0.04 and 0.04, respectively). Urinary 1-OHP positively correlated with the level of 8-oxodGuo and bulky DNA adducts, but not DNA fragmentation. Urinary 1-OHP was a suitable biomarker for an estimate of biologically effective doses of PAH exposure. However, urinary 1-OHP may not be sufficient as a biomarker to assess both cellular and molecular changes of sperm induced by PAHs.
Keywords
Urinalysis; Urine-chemistry; Biomarkers; Cell-alteration; Cell-function; Cellular-function; Cellular-reactions; Analytical-processes; Humans; Men; Reproductive-system; Reproductive-system-disorders; Morphology; Molecular-biology; Molecular-structure; Metallurgy; Exposure-levels; Chromatographic-analysis; Age-groups; Pyrenes; Metabolism; Pathology; DNA-adducts; DNA-damage; Hydroxy-compounds; Author Keywords: 1-Hydroxypyrene; semen quality; DNA integrity; biomarker; 8-oxodGuo; DNA fragmentation
Contact
Hueiwang Anna Jeng, School of Community and Environmental Health, College of Health Sciences, Old Dominion University, 4608 Hampton Boulevard, Health Sciences Building, Room 3140, Norfolk,VA USA
CODEN
JATEF9
CAS No.
5315-79-7
Publication Date
20130101
Document Type
Journal Article
Email Address
hjeng@odu.edu
Funding Type
Grant
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-009504; B20130321
Issue of Publication
2
ISSN
1093-4529
Source Name
Journal of Environmental Science and Health. Part A: Toxic/Hazardous Substances and Environmental Engineering
State
VA
Performing Organization
Old Dominion University
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