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Advanced collector for airborne bioagents.

Han-T; An-H-R; Mainelis-G
Eighth New Jersey Universities Homeland Security Research Consortium Symposium, December 5, 2008, Princeton, New Jersey. Piscataway, NJ: New Jersey Universities Homeland Security Research Consortium, 2008 Dec; :1
Presence of airborne bioagents, including presence of airborne biowarfare agents, is determined by pulling air through a collector, depositing airborne particles onto/into collection substrate and analyzing the substrate for presence of particles of interest. The performance of any collector is defined by its collection efficiency (fraction of airborne particles deposited in the collection substrate) and its concentration rate. The currently available samplers feature concentration rates is approximately ~104-105/min. In this research we designed and tested a radically new and advanced bioagent collector featuring the concentration rate of > 106-107/min. In this sampler the airborne bioagents are electrostatically deposited on a narrow electrode covered with a superhydrophobic material from which they are removed by a tiny (5-40 ÁL) rolling water droplet. The liquid containing particles can then be analyzed by a variety of techniques including microscopy, Polymerase Chain Reaction (PCR) and similar techniques. Our tests with Bacillus anthracis simulants and droplets as small as 5ÁL revealed that the sampler achieved concentration rate of 1.3x106/min ? much higher than existing samplers. The sampler was shown to be able to sustain the efficient collection over prolonged periods of time and was compatible with PCR. The new technology offers an advantage of faster detection of low airborne bioagent concentrations.
Airborne-particles; Airborne-dusts; Biological-agents; Biological-material; Biological-weapons; Analytical-processes; Samplers; Electrostatic-filters; Microscopy; Air-samplers; Air-samples; Air-sampling
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Eighth New Jersey Universities Homeland Security Research Consortium Symposium, December 5, 2008, Princeton, New Jersey
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Rutgers the State of New Jersey - New Brunswick